Adamkiewicz Michal, Rubinsky Boris
Department of Mechanical Engineering, University of California, Berkeley, CA 94720, USA.
Department of Mechanical Engineering, University of California, Berkeley, CA 94720, USA.
Cryobiology. 2015 Dec;71(3):518-21. doi: 10.1016/j.cryobiol.2015.10.152. Epub 2015 Nov 5.
We describe a new cryogenic 3D printing technology for freezing hydrogels, with a potential impact to tissue engineering. We show that complex frozen hydrogel structures can be generated when the 3D object is printed immersed in a liquid coolant (liquid nitrogen), whose upper surface is maintained at the same level as the highest deposited layer of the object. This novel approach ensures that the process of freezing is controlled precisely, and that already printed frozen layers remain at a constant temperature. We describe the device and present results which illustrate the potential of the new technology.
我们描述了一种用于冷冻水凝胶的新型低温3D打印技术，该技术对组织工程可能产生影响。我们表明，当3D物体浸入液体冷却剂（液氮）中进行打印时，可以生成复杂的冷冻水凝胶结构，该液体冷却剂的上表面与物体的最高沉积层保持在同一水平。这种新颖的方法可确保精确控制冷冻过程，并且已打印的冷冻层保持恒定温度。我们介绍了该设备并展示了说明这项新技术潜力的结果。
