CEISAM-UMR CNRS 6230, Université de Nantes, 2 rue de la Houssinière, 44322, Nantes, France.
UFIP-UMR CNRS 6286, Université de Nantes, 2 rue de la Houssinière, 44322, Nantes, France.
Adv Healthc Mater. 2015 Dec 9;4(17):2727-34. doi: 10.1002/adhm.201500562. Epub 2015 Nov 9.
Strongly solvatochromic fluorophores are devised, containing alkyl chains and enable to self-assemble as very bright fluorescent organic nanoparticles (FONs) in water (Φf = 0.28). The alkyl chains impart each fluorophore with strongly hydrophobic surroundings, causing distinct emission colors between FONs where the fluorophores are associated, and their disassembled state. Such color change is harnessed to assess the long-term fate of FONs in both cancer cells and monocytes/macrophages. Disintegration of the orange-emitting FONs by monocytes/macrophages is evidenced through the formation of micrometer green-yellowish emitting vesicles. By contrast, cancer cells retain longer the integrity of organic nanoparticles. In both cases, no significant toxicity is detected, making FONs as valuable bioimaging agents for cell tracking with weak risks of deleterious accumulation and low degradation rate.
设计了具有强烈溶致变色性的荧光团，包含烷基链，能够在水中自组装成非常亮的荧光有机纳米粒子（FONs）（Φf = 0.28）。烷基链赋予每个荧光团强烈的疏水环境，导致 FONs 中荧光团缔合和离解状态之间的发射颜色明显不同。这种颜色变化可用于评估 FONs 在癌细胞和单核细胞/巨噬细胞中的长期命运。单核细胞/巨噬细胞破坏橙色发射的 FONs，形成微米级的黄绿色发射囊泡。相比之下，癌细胞保持有机纳米粒子完整性的时间更长。在这两种情况下，都没有检测到明显的毒性，使得 FONs 成为用于细胞追踪的有价值的生物成像剂，其具有较低的有害积累风险和低降解率。
