小鼠原代培养肌管中体外肌肉收缩模型的评估
Evaluation of an in vitro muscle contraction model in mouse primary cultured myotubes.
作者信息
Manabe Yasuko, Ogino Shinya, Ito Miyuki, Furuichi Yasuro, Takagi Mayumi, Yamada Mio, Goto-Inoue Naoko, Ono Yusuke, Fujii Nobuharu L
机构信息
Department of Health Promotion Sciences, Graduate School of Human Health Sciences, Tokyo Metropolitan University, Hachioji 192-0397, Japan.
Department of Health Promotion Sciences, Graduate School of Human Health Sciences, Tokyo Metropolitan University, Hachioji 192-0397, Japan; Department of Marine Science and Resources, College of Bioresource Sciences, Nihon University, Fujisawa, Kanagawa 252-0880, Japan.
出版信息
Anal Biochem. 2016 Mar 15;497:36-8. doi: 10.1016/j.ab.2015.10.010. Epub 2015 Nov 6.
To construct an in vitro contraction model with the primary cultured myotubes, we isolated satellite cells from the mouse extensor digitorum longus. Differentiated myotubes possessed a greater number of sarcomere assemblies and higher expression levels of myosin heavy chain, cytochrome c oxidase IV, and myoglobin than in C2C12 myotubes. In agreement with these results regarding the sarcomere assemblies and protein expressions, the primary myotubes showed higher contractile activity stimulated by the electric pulses than that in the C2C12 myotubes. These data suggest that mouse primary myotubes will be a valuable research tool as an in vitro muscle contraction model.
为了用原代培养的肌管构建体外收缩模型，我们从小鼠趾长伸肌中分离出卫星细胞。与C2C12肌管相比，分化的肌管拥有更多的肌节组装，并且肌球蛋白重链、细胞色素c氧化酶IV和肌红蛋白的表达水平更高。与这些关于肌节组装和蛋白质表达的结果一致，原代肌管在电脉冲刺激下显示出比C2C12肌管更高的收缩活性。这些数据表明，小鼠原代肌管作为体外肌肉收缩模型将是一种有价值的研究工具。
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