γ-氨基丁酸对麻醉大鼠血压和血液动力学的影响。
Effect of GABA on blood pressure and blood dynamics of anesthetic rats.
作者信息
Ma Pengju, Li Ting, Ji Fanceng, Wang Haibo, Pang Juntao
机构信息
Department of Anesthesiogy, Anqiu People's Hospital Anqiu 262100, China.
Delivery Room, People's Hospital of Anqiu Anqiu 262100, China.
出版信息
Int J Clin Exp Med. 2015 Aug 15;8(8):14296-302. eCollection 2015.
BACKGROUND
This study aimed to investigate GABA effects on blood pressure and blood dynamics of anesthetic rats by observing spontaneously hypertensive rats under both anesthesia and waking state.
MATERIALS AND METHODS
72 male waking Wistar-Kyokos (WKY) rats and 72 male anesthetized spontaneously hypertensive (SHR) rats were randomly divided into control group and experimental group (N = 36 each). Rats were further divided into three subgroups (N = 12 each), which received 15 μmol GABA, 35 nmol muscimol, or 4 nmol dicentrine into unilateral paraventricular nucleus, respectively. Rats in the control group (WKY1) and experimental group (SHR1) were compared for the GABA effect on blood pressure (MAP), heart rate (HR), and arterial baroreceptor reflex function (BRS) changes under waking state. Anesthetic WKY rats (WKY2) and spontaneously hypertensive rats (SHR2) were compared for the GABA effect on those abovementioned indexes. Abdominal aorta mean arterial pressure, heart rate, and arterial baroreceptor reflex function changes were compared in all rats.
RESULTS
MAP, HR, and BRS were slightly lower in the rats under anesthetic state than in waking state before treatment (P < 0.05); they did not show significant changes between anesthetic and waking state, however, after treatment (P > 0.05). Unilateral paraventricular nucleus injection of GABA or muscimol elevated MAP, HR, and BRS in both normal and spontaneously hypertensive rats under waking or anesthetic state (P < 0.05). In addition, the amplitudes of changes of three indicators in spontaneously hypertensive group were markedly higher than those of control group (P < 0.05). Dicentrine could induce MAP and HR to increase, while BRS decreased significantly (P < 0.05). The amplitudes of changes in spontaneously hypertensive group were larger than those of normal group (P < 0.05).
CONCLUSION
Centrally GABA injection can enhance the BRS function in spontaneously hypertensive rats and adjust heart rate to reduce the blood pressure fluctuation. It may play a role in reducing blood pressure and protecting cardiovascular function.
背景
本研究旨在通过观察麻醉和清醒状态下的自发性高血压大鼠，探讨γ-氨基丁酸（GABA）对麻醉大鼠血压和血流动力学的影响。
材料与方法
将72只雄性清醒的Wistar-Kyoto（WKY）大鼠和72只雄性麻醉的自发性高血压（SHR）大鼠随机分为对照组和实验组（每组N = 36只）。大鼠进一步分为三个亚组（每组N = 12只），分别向单侧室旁核注射15 μmol GABA、35 nmol蝇蕈醇或4 nmol汉防己甲素。比较对照组（WKY1）和实验组（SHR1）大鼠在清醒状态下GABA对血压（MAP）、心率（HR）和动脉压力感受器反射功能（BRS）变化的影响。比较麻醉的WKY大鼠（WKY2）和自发性高血压大鼠（SHR2）上述指标的GABA效应。比较所有大鼠腹主动脉平均动脉压、心率和动脉压力感受器反射功能的变化。
结果
治疗前，麻醉状态下大鼠的MAP、HR和BRS略低于清醒状态（P < 0.05）；然而，治疗后麻醉和清醒状态之间未显示出显著变化（P > 0.05）。在清醒或麻醉状态下，单侧室旁核注射GABA或蝇蕈醇可使正常和自发性高血压大鼠的MAP、HR和BRS升高（P < 0.05）。此外，自发性高血压组三项指标的变化幅度明显高于对照组（P < 0.05）。汉防己甲素可诱导MAP和HR升高，而BRS显著降低（P < 0.05）。自发性高血压组的变化幅度大于正常组（P < 0.05）。
结论
中枢注射GABA可增强自发性高血压大鼠的BRS功能，并调节心率以减少血压波动。它可能在降低血压和保护心血管功能方面发挥作用。
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