嗜热四膜虫端粒酶p75-p45-p19亚复合物是一种独特的CST复合物。
The Tetrahymena telomerase p75-p45-p19 subcomplex is a unique CST complex.
作者信息
Wan Bingbing, Tang Ting, Upton Heather, Shuai Jin, Zhou Yuanzhe, Li Song, Chen Juan, Brunzelle Joseph S, Zeng Zhixiong, Collins Kathleen, Wu Jian, Lei Ming
机构信息
National Center for Protein Science Shanghai, State Key Laboratory of Molecular Biology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, China.
Shanghai Science Research Center, Chinese Academy of Sciences, Shanghai, China.
出版信息
Nat Struct Mol Biol. 2015 Dec;22(12):1023-6. doi: 10.1038/nsmb.3126. Epub 2015 Nov 9.
Abstract
Tetrahymena telomerase holoenzyme subunits p75, p45 and p19 form a subcomplex (7-4-1) peripheral to the catalytic core. We report structures of p45 and p19 and reveal them as the Stn1 and Ten1 subunits of the CST complex, which stimulates telomerase complementary-strand synthesis. 7-4-1 binds telomeric single-stranded DNA, and mutant p19 overexpression causes telomere 3'-overhang elongation. We propose that telomerase-tethered Tetrahymena CST coordinates telomere G-strand and C-strand synthesis.
摘要
嗜热四膜虫端粒酶全酶亚基p75、p45和p19形成一个位于催化核心外围的亚复合物（7-4-1）。我们报道了p45和p19的结构，并揭示它们是CST复合物的Stn1和Ten1亚基，该复合物可刺激端粒酶互补链的合成。7-4-1结合端粒单链DNA，突变型p19的过表达导致端粒3'端悬突延长。我们提出，与端粒酶相连的嗜热四膜虫CST可协调端粒G链和C链的合成。
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