斑马鱼中包括cUMP在内的环核苷酸（cNMPs）的时间和器官特异性检测。
Temporal and organ-specific detection of cNMPs including cUMP in the zebrafish.
作者信息
Dittmar Fanni, Abdelilah-Seyfried Salim, Tschirner Sarah K, Kaever Volkhard, Seifert Roland
机构信息
Institute of Pharmacology, Hannover Medical School, Carl-Neuberg-Str. 1, D-30625 Hannover, Germany.
Institute of Biochemistry and Biology, University of Potsdam, Karl-Liebknecht-Str. 24-25, D-14476 Potsdam, Germany; Institute of Molecular Biology, Hannover Medical School, Carl-Neuberg-Str. 1, D-30625 Hannover, Germany.
出版信息
Biochem Biophys Res Commun. 2015 Dec 25;468(4):708-12. doi: 10.1016/j.bbrc.2015.11.020. Epub 2015 Nov 6.
The cyclic pyrimidine nucleotides cCMP and cUMP occur in mammalian cell lines. Recently, cCMP was also identified in mouse organs. Due to technical difficulties, it has not been possible to detect cUMP in organs or tissues yet. Here, we have generated a temporal profile of the occurrence of nucleoside 3',5'-cyclic monophosphates during different developmental stages of embryogenesis and in different organs of the adult zebrafish Danio rerio. Cyclic nucleotides were quantified by high performance liquid chromatography quadrupole tandem mass spectrometry. The identity of cCMP and cUMP in the zebrafish was confirmed by high performance liquid chromatography quadrupole time-of-flight mass spectrometry. We show for the first time that cUMP can be detected during embryogenesis and in adult organs of this vertebrate model system.
环状嘧啶核苷酸cCMP和cUMP存在于哺乳动物细胞系中。最近，cCMP在小鼠器官中也被鉴定出来。由于技术困难，目前还无法在器官或组织中检测到cUMP。在此，我们绘制了斑马鱼胚胎发育不同阶段以及成年斑马鱼不同器官中核苷3',5'-环一磷酸出现情况的时间分布图。通过高效液相色谱-四极杆串联质谱对环状核苷酸进行定量分析。利用高效液相色谱-四极杆飞行时间质谱确认了斑马鱼中cCMP和cUMP的身份。我们首次表明，在这个脊椎动物模型系统的胚胎发育过程和成年器官中可以检测到cUMP。
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