Faculty of Natural Sciences and Engineering, Tampere University, FI-33101 Tampere, Finland.
Natural Resources Institute Finland (Luke), FI-00791 Helsinki, Finland.
Molecules. 2019 Jun 25;24(12):2330. doi: 10.3390/molecules24122330.
Endophytes are microorganisms living inside plant hosts and are known to be beneficial for the host plant vitality. In this study, we isolated three endophytic fungus species from the roots of Scots pine seedlings growing on Finnish drained peatland setting. The isolated fungi belonged to dark septate endophytes (DSE). The metabolic profiles of the hot water extracts of the fungi were investigated using Ultrahigh Performance Liquid Chromatography with Diode Array Detection and Electron Spray Ionization source Mass Spectrometry with Orbitrap analyzer (UPLC-DAD-ESI-MS-Orbitrap). Out of 318 metabolites, we were able to identify 220, of which a majority was amino acids and peptides. Additionally, opine amino acids, amino acid quinones, Amadori compounds, cholines, nucleobases, nucleosides, nucleotides, siderophores, sugars, sugar alcohols and disaccharides were found, as well as other previously reported metabolites from plants or endophytes. Some differences of the metabolic profiles, regarding the amount and identity of the found metabolites, were observed even though the fungi were isolated from the same host. Many of the discovered metabolites have been described possessing biological activities and properties, which may make a favorable contribution to the host plant nutrient availability or abiotic and biotic stress tolerance.
内生菌是生活在植物宿主内部的微生物，已知对宿主植物的活力有益。在这项研究中，我们从生长在芬兰排水泥炭地上的苏格兰松幼苗根部分离出三种内生真菌。分离出的真菌属于深色隔孢菌素（DSE）。使用带有二极管阵列检测和电喷雾电离源质量光谱仪与轨道阱分析器的超高效液相色谱法（UPLC-DAD-ESI-MS-Orbitrap）研究了真菌热水提取物的代谢谱。在 318 种代谢物中，我们能够鉴定出 220 种，其中大多数是氨基酸和肽。此外，还发现了氨基酸、氨基酸醌、阿马多里化合物、胆碱、碱基、核苷、核苷酸、铁载体、糖、糖醇和二糖，以及其他先前从植物或内生菌中报道的代谢物。尽管真菌是从同一宿主中分离出来的，但观察到代谢谱的一些差异，包括发现的代谢物的数量和种类。许多发现的代谢物具有生物活性和特性，这可能对宿主植物的养分可用性或非生物和生物胁迫耐受性做出有利贡献。
