Brem G, Springmann K
Lehrstuhl für Molekulare Tierzucht, Ludwig-Maximilians-Universität München.
Tierarztl Prax Suppl. 1989;4:31-4.
The techniques for the production of transgenic pigs are described. Once a gene interesting for animal breeding is found, a gene construct is cloned. The gene construct is a combination of a structure gene and a promoter region which regulates the gene expression of the transgene. The DNA solution is microinjected into the pronuclei of fertilized ova. The injected ova are transferred to synchronised recipient pigs. The animals born must be controlled for the integration of the injected gene construct. In transgenic offspring the expression of the gene, the heredity to the next generation and the biological activity have to be tested. The overall efficiency of a gene transfer programme in pig is about 0.5%. This is lower than in similar projects in mice.
本文描述了转基因猪的生产技术。一旦发现对动物育种有意义的基因，就克隆一个基因构建体。该基因构建体是结构基因和调控转基因基因表达的启动子区域的组合。将DNA溶液显微注射到受精卵的原核中。将注射后的卵转移到同步化的受体猪中。出生的动物必须检查注射的基因构建体是否整合。对于转基因后代，必须测试基因的表达、向下一代的遗传传递以及生物活性。猪基因转移计划的总体效率约为0.5%。这低于小鼠的类似项目。
