Serov O L
Ontogenez. 1985 Nov-Dec;16(6):553-67.
A review of literature on genetic transformation of animal (mouse and Drosophila) germ cells by microinjection of the cloned genes into the pronuclei or into the early embryos. The fate of foreign genes and mechanisms of their integration into the recipient genome are considered in detail, as well as genetic consequences of the exogenic DNA insertion. Expression of the donor genes in transgenic animals is analyzed, its irregular and unusual pattern is noted in transgenic mice. An attempt has been undertaken not only to stress obvious advances in genetic transformation of animals, but also to outline unsolved problems related to inheritance of exogenous genes, insertion mutagenesis and irregular expression of the donor genes.
一篇关于通过将克隆基因显微注射到原核或早期胚胎中来对动物（小鼠和果蝇）生殖细胞进行遗传转化的文献综述。详细探讨了外源基因的命运及其整合到受体基因组中的机制，以及外源DNA插入的遗传后果。分析了转基因动物中供体基因的表达情况，注意到转基因小鼠中存在不规则和异常的表达模式。不仅试图强调动物遗传转化方面的明显进展，还概述了与外源基因遗传、插入诱变和供体基因不规则表达相关的未解决问题。
