D型肉毒梭菌神经毒素：纯化与检测

Clostridium botulinum type D neurotoxin: purification and detection.

作者信息

De Jongh K S, Schwartzkoff C L, Howden M E

机构信息

School of Chemistry, Macquarie University, North Ryde, Australia.

出版信息

Toxicon. 1989;27(2):221-8. doi: 10.1016/0041-0101(89)90135-9.

DOI:10.1016/0041-0101(89)90135-9

PMID:2655181

Abstract

A method is reported for the purification of type D botulinum toxin using a combination of low and high pressure ion exchange chromatography. The procedure produced homogeneous toxin in its free form in 3 days, with a specific toxicity in mice of 5.4 x 10(7) LD50/mg protein. Polyclonal antibodies against the pure toxin were raised in rabbits and detected the toxin in both ELISA and western blotting. The antibodies also detected type C1 botulinum toxin using these techniques, confirming the presence of cross-reacting antigenic determinants in these two proteins.

摘要

报道了一种使用低压和高压离子交换色谱相结合的方法来纯化D型肉毒杆菌毒素。该程序在3天内产生了游离形式的纯毒素，其在小鼠中的比毒性为5.4×10⁷ LD50/毫克蛋白质。用纯毒素对兔进行免疫以产生多克隆抗体，这些抗体在ELISA和蛋白质印迹法中均能检测到该毒素。这些抗体还使用这些技术检测到C1型肉毒杆菌毒素，证实了这两种蛋白质中存在交叉反应性抗原决定簇。