RNASEL基因座中马血白细胞的年龄相关甲基化图谱。
Age-related methylation profiles of equine blood leukocytes in the RNASEL locus.
作者信息
Ząbek T, Semik E, Szmatoła T, Oklejewicz B, Fornal A, Bugno-Poniewierska M
机构信息
National Research Institute of Animal Production, Krakowska 1, 32-083, Balice, Poland.
Centre of Applied Biotechnology and Basic Sciences, University of Rzeszow, Sokołowska 26, 36-100, Kolbuszowa, Poland.
出版信息
J Appl Genet. 2016 Aug;57(3):383-8. doi: 10.1007/s13353-015-0323-4. Epub 2015 Nov 9.
Methylation profiles across three CpG islands of the RNASEL gene were determined in blood leukocyte samples of Anglo-Arabian and Hucul horses. Bisulfite sequencing revealed hypomethylated state of the RNASEL promoter coinciding with methylated CpG island placed inside the gene. Several CpG sites were identified for which the methylation state was influenced by DNA polymorphism. Two of them showed monoallelic methylation. One of the CpG sites revealed functional polymorphism. A number of partially methylated CpG sites have been observed in the promoter area of RNASEL, which were used for the comparison of breed- and age-related effects. Clone bisulfite sequencing of blood leukocyte samples collected at different ages from particular individuals of AA and HC breeds and, also, BSPCR sequencing of 50 samples of juvenile and old AA and HC horses revealed increased methylation in particular CpG sites during aging. The age-related heterogeneity of white blood cells was hypothesized as being one of the potential causes of observed variability of methylation profiles in the RNASEL promoter.
在盎格鲁 - 阿拉伯马和胡苏尔马的血液白细胞样本中，测定了RNASEL基因三个CpG岛的甲基化谱。亚硫酸氢盐测序显示，RNASEL启动子处于低甲基化状态，同时基因内部的CpG岛发生甲基化。鉴定出了几个受DNA多态性影响甲基化状态的CpG位点。其中两个显示单等位基因甲基化。其中一个CpG位点表现出功能多态性。在RNASEL的启动子区域观察到许多部分甲基化的CpG位点，用于比较品种和年龄相关的影响。对来自AA和HC品种特定个体不同年龄采集的血液白细胞样本进行克隆亚硫酸氢盐测序，以及对50匹幼年和老年AA和HC马的样本进行BSPCR测序，结果显示在衰老过程中特定CpG位点的甲基化增加。白细胞与年龄相关的异质性被认为是RNASEL启动子甲基化谱观察到的变异性的潜在原因之一。
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