Fansa Eyad Kalawy, O'Reilly Nicola J, Ismail Shehab, Wittinghofer Alfred
Max Planck Institute of Molecular Physiology, Dortmund, Germany.
The Francis Crick Institute, London, UK.
EMBO Rep. 2015 Dec;16(12):1583-5. doi: 10.15252/embr.201541404. Epub 2015 Nov 9.
This study shows that the prenylated C‐terminus of RPGR can bind to PDE6δ with high affinity, suggesting two distinct binding sites of the RPGR/PDE6δ complex. The serine residue at the −3 position relative to the prenylated cysteine seems to play a key role in defining the selectivity of PDE6δ towards ciliary prenylated cargo. [Image: see text]
本研究表明,RPGR的异戊烯化C末端可与PDE6δ高亲和力结合,提示RPGR/PDE6δ复合物存在两个不同的结合位点。相对于异戊烯化半胱氨酸在−3位置的丝氨酸残基,似乎在决定PDE6δ对纤毛异戊烯化货物的选择性方面起关键作用。[见图]