异戊烯基结合蛋白PrBP/δ：一种参与细胞内运输的伴侣蛋白。
The prenyl-binding protein PrBP/δ: a chaperone participating in intracellular trafficking.
作者信息
Zhang Houbin, Constantine Ryan, Frederick Jeanne M, Baehr Wolfgang
机构信息
Department of Ophthalmology, John A. Moran Eye Center, University of Utah Health Science Center, 65 Mario Capecchi Dr., Salt Lake City, UT 84132, USA.
出版信息
Vision Res. 2012 Dec 15;75:19-25. doi: 10.1016/j.visres.2012.08.013. Epub 2012 Aug 29.
Abstract
Expressed ubiquitously, PrBP/δ functions as chaperone/co-factor in the transport of a subset of prenylated proteins. PrBP/δ features an immunoglobulin-like β-sandwich fold for lipid binding, and interacts with diverse partners. PrBP/δ binds both C-terminal C15 and C20 prenyl side chains of phototransduction polypeptides and small GTP-binding (G) proteins of the Ras superfamily. PrBP/δ also interacts with the small GTPases, ARL2 and ARL3, which act as release factors (GDFs) for prenylated cargo. Targeted deletion of the mouse Pde6d gene encoding PrBP/δ resulted in impeded trafficking to the outer segments of GRK1 and cone PDE6 which are predicted to be farnesylated and geranylgeranylated, respectively. Rod and cone transducin trafficking was largely unaffected. These trafficking defects produce progressive cone-rod dystrophy in the Pde6d(-/-) mouse.
摘要
PrBP/δ在全身广泛表达，在一组异戊二烯化蛋白的转运过程中作为伴侣蛋白/辅助因子发挥作用。PrBP/δ具有用于脂质结合的免疫球蛋白样β-折叠结构，并与多种蛋白相互作用。PrBP/δ既能结合光转导多肽的C末端C15和C20异戊二烯侧链，也能结合Ras超家族的小GTP结合（G）蛋白。PrBP/δ还与小GTP酶ARL2和ARL3相互作用，这两种酶作为异戊二烯化货物的释放因子（GDF）。对编码PrBP/δ的小鼠Pde6d基因进行靶向缺失，导致预计分别被法尼基化和香叶基香叶基化的GRK1和视锥细胞PDE6向视杆细胞外段的转运受阻。视杆细胞和视锥细胞转导素的转运在很大程度上未受影响。这些转运缺陷在Pde6d（-/-）小鼠中导致进行性视锥-视杆营养不良。
相似文献
1
The prenyl-binding protein PrBP/δ: a chaperone participating in intracellular trafficking.异戊烯基结合蛋白PrBP/δ：一种参与细胞内运输的伴侣蛋白。
Vision Res. 2012 Dec 15;75:19-25. doi: 10.1016/j.visres.2012.08.013. Epub 2012 Aug 29.
2
Deletion of PrBP/delta impedes transport of GRK1 and PDE6 catalytic subunits to photoreceptor outer segments.PrBP/delta 的缺失会阻碍 GRK1 和 PDE6 催化亚基向光感受器外段的转运。
Proc Natl Acad Sci U S A. 2007 May 22;104(21):8857-62. doi: 10.1073/pnas.0701681104. Epub 2007 May 11.
3
Evaluation of the 17-kDa prenyl-binding protein as a regulatory protein for phototransduction in retinal photoreceptors.评估17-kDa异戊烯基结合蛋白作为视网膜光感受器光转导调节蛋白的作用。
J Biol Chem. 2005 Jan 14;280(2):1248-56. doi: 10.1074/jbc.M410475200. Epub 2004 Oct 25.
4
Membrane protein transport in photoreceptors: the function of PDEδ: the Proctor lecture.光感受器中的膜蛋白转运：PDEδ的功能：普罗克特讲座
Invest Ophthalmol Vis Sci. 2014 Dec 30;55(12):8653-66. doi: 10.1167/iovs.14-16066.
5
Assay and functional properties of PrBP(PDEdelta), a prenyl-binding protein interacting with multiple partners.与多种伴侣相互作用的异戊二烯结合蛋白PrBP（PDEδ）的检测及功能特性
Methods Enzymol. 2005;403:42-56. doi: 10.1016/S0076-6879(05)03005-3.
6
Unc119 gene deletion partially rescues the GRK1 transport defect of Pde6d (- /-) cones.Unc119 基因缺失部分挽救了 Pde6d（-/-）锥体 GRK1 转运缺陷。
Adv Exp Med Biol. 2014;801:487-93. doi: 10.1007/978-1-4614-3209-8_62.
7
Mistrafficking of prenylated proteins causes retinitis pigmentosa 2.异戊二烯化蛋白的错误转运导致色素性视网膜炎2型。
FASEB J. 2015 Mar;29(3):932-42. doi: 10.1096/fj.14-257915. Epub 2014 Nov 24.
8
A model for transport of membrane-associated phototransduction polypeptides in rod and cone photoreceptor inner segments.视杆和视锥光感受器内节中膜相关光转导多肽的转运模型。
Vision Res. 2008 Feb;48(3):442-52. doi: 10.1016/j.visres.2007.08.020. Epub 2007 Oct 18.
9
Mechanistic insights into the role of prenyl-binding protein PrBP/δ in membrane dissociation of phosphodiesterase 6.对异戊二烯结合蛋白PrBP/δ在磷酸二酯酶6膜解离中作用的机制性见解。
Nat Commun. 2018 Jan 8;9(1):90. doi: 10.1038/s41467-017-02569-y.
10
The Function of Arf-like Proteins ARL2 and ARL3 in Photoreceptors.Arf样蛋白ARL2和ARL3在光感受器中的功能。
Adv Exp Med Biol. 2016;854:655-61. doi: 10.1007/978-3-319-17121-0_87.
引用本文的文献
1
Geranylgeranylated SCF regulates selective outer mitochondrial membrane proteostasis and function.香叶基香叶酰化 SCF 调节选择性的外线粒体膜蛋白质稳态和功能。
Cell Rep. 2024 Oct 22;43(10):114783. doi: 10.1016/j.celrep.2024.114783. Epub 2024 Sep 21.
2
Updates on protein-prenylation and associated inherited retinopathies.蛋白质异戊二烯化及相关遗传性视网膜病变的研究进展
Front Ophthalmol (Lausanne). 2024 Jul 4;4:1410874. doi: 10.3389/fopht.2024.1410874. eCollection 2024.
3
Geranylgeranylated-SCF Regulates Selective Outer Mitochondrial Membrane Proteostasis and Function.香叶基香叶酰化的SCF调节线粒体外膜的选择性蛋白质稳态和功能。
bioRxiv. 2024 Apr 16:2024.04.16.589745. doi: 10.1101/2024.04.16.589745.
4
DFT and molecular simulation validation of the binding activity of PDEδ inhibitors for repression of oncogenic k-Ras.基于密度泛函理论和分子模拟验证 PDEδ 抑制剂对致癌性 k-Ras 的抑制作用的结合活性。
PLoS One. 2024 Mar 8;19(3):e0300035. doi: 10.1371/journal.pone.0300035. eCollection 2024.
5
Palmitoylation and PDE6δ regulate membrane-compartment-specific substrate ubiquitylation and degradation.棕榈酰化和 PDE6δ 调节膜区室特异性底物泛素化和降解。
Cell Rep. 2023 Jan 31;42(1):111999. doi: 10.1016/j.celrep.2023.111999. Epub 2023 Jan 19.
6
The ARF GAPs ELMOD1 and ELMOD3 act at the Golgi and cilia to regulate ciliogenesis and ciliary protein traffic.ARF GAPs ELMOD1 和 ELMOD3 在高尔基体和纤毛上发挥作用，调节纤毛发生和纤毛蛋白运输。
Mol Biol Cell. 2022 Feb 1;33(2):ar13. doi: 10.1091/mbc.E21-09-0443. Epub 2021 Nov 24.
7
Deletion of the phosphatase INPP5E in the murine retina impairs photoreceptor axoneme formation and prevents disc morphogenesis.在鼠的视网膜中删除磷酸酶 INPP5E 会损害光感受器轴突的形成并阻止盘状结构的形成。
J Biol Chem. 2021 Jan-Jun;296:100529. doi: 10.1016/j.jbc.2021.100529. Epub 2021 Mar 10.
8
An intrinsic compartmentalization code for peripheral membrane proteins in photoreceptor neurons.光感受器神经元中外周膜蛋白的固有区室化代码。
J Cell Biol. 2019 Nov 4;218(11):3753-3772. doi: 10.1083/jcb.201906024. Epub 2019 Oct 8.
9
Balancing the Photoreceptor Proteome: Proteostasis Network Therapeutics for Inherited Retinal Disease.平衡光感受器蛋白质组：用于遗传性视网膜疾病的蛋白质稳态网络治疗。
Genes (Basel). 2019 Jul 24;10(8):557. doi: 10.3390/genes10080557.
10
Modifying Rap1-signalling by targeting Pde6δ is neuroprotective in models of Alzheimer's disease.靶向 Pde6δ 修饰 Rap1 信号传导对阿尔茨海默病模型具有神经保护作用。
Mol Neurodegener. 2018 Sep 26;13(1):50. doi: 10.1186/s13024-018-0283-3.
本文引用的文献
1
Uncoordinated (UNC)119: coordinating the trafficking of myristoylated proteins.不协调蛋白119：协调豆蔻酰化蛋白的运输
Vision Res. 2012 Dec 15;75:26-32. doi: 10.1016/j.visres.2012.08.012. Epub 2012 Sep 19.
2
The GDI-like solubilizing factor PDEδ sustains the spatial organization and signalling of Ras family proteins.GDI 样溶解因子 PDEδ 维持 Ras 家族蛋白的空间组织和信号转导。
Nat Cell Biol. 2011 Dec 18;14(2):148-58. doi: 10.1038/ncb2394.
3
Arl2-GTP and Arl3-GTP regulate a GDI-like transport system for farnesylated cargo.Arl2-GTP 和 Arl3-GTP 调节法尼基化货物的 GDI 样转运系统。
Nat Chem Biol. 2011 Oct 16;7(12):942-9. doi: 10.1038/nchembio.686.
4
UNC119 is required for G protein trafficking in sensory neurons.UNC119 是感觉神经元中 G 蛋白运输所必需的。
Nat Neurosci. 2011 Jun 5;14(7):874-80. doi: 10.1038/nn.2835.
5
RAS-converting enzyme 1-mediated endoproteolysis is required for trafficking of rod phosphodiesterase 6 to photoreceptor outer segments.RAS 转换酶 1 介导的内切作用对于视杆磷酸二酯酶 6 向光感受器外节的运输是必需的。
Proc Natl Acad Sci U S A. 2011 May 24;108(21):8862-6. doi: 10.1073/pnas.1103627108. Epub 2011 May 9.
6
Analysis of protein prenylation in vitro and in vivo using functionalized phosphoisoprenoids.使用功能化磷酸异戊二烯类化合物对体外和体内蛋白质异戊二烯化进行分析。
Curr Protoc Protein Sci. 2010 Nov;Chapter 14:Unit14.3. doi: 10.1002/0471140864.ps1403s62.
7
Phosphodiesterase 6 subunits are expressed and altered in idiopathic pulmonary fibrosis.磷酸二酯酶 6 亚基在特发性肺纤维化中表达和改变。
Respir Res. 2010 Oct 27;11(1):146. doi: 10.1186/1465-9921-11-146.
8
Structural characterization of the rod cGMP phosphodiesterase 6.杆状 cGMP 磷酸二酯酶 6 的结构特征。
J Mol Biol. 2010 Aug 20;401(3):363-73. doi: 10.1016/j.jmb.2010.06.044. Epub 2010 Jul 1.
9
The chemical toolbox for monitoring protein fatty acylation and prenylation.蛋白质酰化和类异戊二烯化的化学工具包。
Nat Chem Biol. 2010 Jul;6(7):498-506. doi: 10.1038/nchembio.388.
10
Understanding and exploiting protein prenyltransferases.了解和利用蛋白质异戊二烯基转移酶。
Chembiochem. 2010 Jun 14;11(9):1194-201. doi: 10.1002/cbic.200900727.
Zotero 插件