Kim Hyunjung, Kim Yonggoo, Lee Hae Kyung
Clin Lab. 2015;61(9):1337-40. doi: 10.7754/clin.lab.2015.150231.
In the present study, the influence of preanalytical variables on prothrombin time (PT), activated partial thromboplastin time (aPTT), and fibrinogen testing is investigated.
Samples collected from 104 patients were used for the study. PT, aPTT, and fibrinogen levels were tested with 50%-filled tubes to investigate the influence of sample volume. We measured the same parameters immediately after sampling, after 6 hours, and after 24 hours to investigate the influence of storage time. Samples were also tested after 6 hours at 4 degrees C to investigate the influence of storage temperature.
Use of 50%-filled tubes did not alter the results of PT and fibrinogen testing, but caused a significant bias to the aPTT test. The results of aPTT at 24 hours were also significantly different from results immediately after sampling. However, uncentrifuged citrated samples stored for 24 hours at room temperature may still be suitable for PT and fibrinogen testing. Incubation at 4 degrees C for up to 6 hours did not bias the results of PT, aPTT, or fibrinogen testing.
Whilst aPTT is malleable to preanalytical variables, PT and fibrinogen showed stable results across variations in sample volume, storage time, and temperature.
在本研究中，调查了分析前变量对凝血酶原时间（PT）、活化部分凝血活酶时间（aPTT）和纤维蛋白原检测的影响。
从104例患者采集的样本用于本研究。使用50%充盈度的试管检测PT、aPTT和纤维蛋白原水平，以研究样本量的影响。我们在采样后立即、6小时后和24小时后测量相同参数，以研究储存时间的影响。样本还在4℃下保存6小时后进行检测，以研究储存温度的影响。
使用50%充盈度的试管不会改变PT和纤维蛋白原检测结果，但会导致aPTT检测出现显著偏差。24小时时的aPTT结果也与采样后立即得到的结果有显著差异。然而，未离心的枸橼酸盐样本在室温下储存24小时仍可能适用于PT和纤维蛋白原检测。在4℃下孵育长达6小时不会使PT、aPTT或纤维蛋白原检测结果产生偏差。
虽然aPTT易受分析前变量影响，但PT和纤维蛋白原在样本量、储存时间和温度变化时结果稳定。
