Tang L C, Cotzias G C
Proc Natl Acad Sci U S A. 1977 Mar;74(3):1242-4. doi: 10.1073/pnas.74.3.1242.
We obtained 12 groups of mice with widely different neurological responses to levodopa by selecting them from different strains and submitting some of them to pretreatments. We scored the symptoms evoked by a standardized dose of levodopa in one subgroup from each group. We tested another subgroup for activation of an adenylate cyclase [ATP pyrophosphate-lyase (cyclizing), EC 4.6.1.1] by a standardized dose of dopamine added to homogenates of the caudate nuclei of the brains of these mice. Both sets of tests were performed randomly. When the accruing two sets of data were plotted against each other there emerged a straight line which fitted the data with a coefficient of correlation of 0.97 (P less than 0.0001). The dopamine-dependent activity of the adenylate cyclase of the brain was thus shown to be a determinant of the neurological responses of intact animals to a dopaminergic drug.
我们从不同品系中挑选小鼠,并对其中一些进行预处理,从而获得了12组对左旋多巴有广泛不同神经反应的小鼠。我们对每组中的一个亚组给予标准化剂量的左旋多巴,并对所引发的症状进行评分。我们对另一个亚组进行测试,向这些小鼠大脑尾状核匀浆中添加标准化剂量的多巴胺,以检测腺苷酸环化酶[ATP焦磷酸裂解酶(环化),EC 4.6.1.1]的激活情况。两组测试均随机进行。当将累积的两组数据相互绘制时,出现了一条直线,该直线与数据拟合,相关系数为0.97(P小于0.0001)。因此,大脑腺苷酸环化酶的多巴胺依赖性活性被证明是完整动物对多巴胺能药物神经反应的一个决定因素。