金黄色葡萄球菌中莫匹罗星耐药性的筛查

Screening for Mupirocin Resistance in Staphylococcus.

作者信息

Sanju Avr Jeya, Kopula Sridharan Sathyamoorthy, Palraj Kennedy Kumar

机构信息

Student, Department of Microbiology, Sri Ramachandra Medical College and Research Institute, Sri Ramachandra University , Porur, Chennai, India .

Associate Professor, Department of Microbiology, Sri Ramachandra Medical College and Research Institute, Sri Ramachandra University , Porur, Chennai, India .

出版信息

J Clin Diagn Res. 2015 Oct;9(10):DC09-10. doi: 10.7860/JCDR/2015/15230.6678. Epub 2015 Oct 1.

DOI:10.7860/JCDR/2015/15230.6678

PMID:26557517

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4625236/

Abstract

INTRODUCTION

Mupirocin is widely used topical antibiotic for the treatment of skin and soft tissue infections caused by Staphylococcus and Streptococcus. In addition nasal formulations are approved for the use in nasal eradication of methicillin-resistant Staphylococcus aureus in patients and health care workers. Wide usage of mupirocin has resulted in resistance leading to treatment failure.

AIM

To screen for the mupirocin resistance among the Staphylococcus isolates using disc diffusion and minimum inhibitory concentration method.

MATERIALS AND METHODS

A cross-sectional study was done at Microbiology Department of Sri Ramachandra University with 100 strains of Staphylococcus spp isolated from skin and soft tissue infections. Methicillin susceptibility was done by disc diffusion method using oxacillin (1 μgm) and cefoxitin (30 μgm) discs. Isolates were screened for mupirocin resistance by disc diffusion method using 5 μgm discs. High level and low level resistance determined by MIC using agar dilution method.

RESULTS

In 100 Staphylococcus spp 56 were Staphylococcus aureus and 44 were CoNS. Among the 56 Staphylococcus aureus 49 (87.5%) were mupirocin susceptible and 7 (12.5%) resistant by 5μg disc diffusion method. However by MIC method 11 (19.6%) were high and low level mupirocin resistant. Out of 44 CoNS 22 (50%) and 18 (41%) were susceptible by disc diffusion and MIC method respectively. Of the 26 resistant CoNS low level and high level mupirocin resistant was observed in 7 (15.9%) and 19 (43.1%) respectively.

CONCLUSION

Screening for mupirocin resistance by disc diffusion method is important before attempting decolonisation. Mupirocin resistance is more with CoNS. Disc diffusion method may miss low level Mupirocin resistance.

摘要

引言

莫匹罗星是一种广泛使用的外用抗生素，用于治疗由葡萄球菌和链球菌引起的皮肤及软组织感染。此外，鼻腔制剂已被批准用于患者和医护人员鼻腔根除耐甲氧西林金黄色葡萄球菌。莫匹罗星的广泛使用导致了耐药性，进而导致治疗失败。

目的

采用纸片扩散法和最低抑菌浓度法筛选葡萄球菌分离株中的莫匹罗星耐药性。

材料与方法

在斯里兰卡拉马钱德拉大学微生物学系进行了一项横断面研究，从皮肤和软组织感染中分离出100株葡萄球菌属菌株。采用纸片扩散法，使用苯唑西林（1μg）和头孢西丁（30μg）纸片检测甲氧西林敏感性。使用5μg纸片通过纸片扩散法筛选分离株的莫匹罗星耐药性。采用琼脂稀释法通过最低抑菌浓度确定高水平和低水平耐药性。

结果

在100株葡萄球菌属菌株中，56株为金黄色葡萄球菌，44株为凝固酶阴性葡萄球菌（CoNS）。在56株金黄色葡萄球菌中，通过5μg纸片扩散法，49株（87.5%）对莫匹罗星敏感，7株（12.5%）耐药。然而，通过最低抑菌浓度法，11株（19.6%）为高水平和低水平莫匹罗星耐药。在44株CoNS中，分别有22株（50%）和18株（41%）通过纸片扩散法和最低抑菌浓度法敏感。在26株耐药CoNS中，分别有7株（15.9%）和19株（43.1%）观察到低水平和高水平莫匹罗星耐药。