Zhang Jinjuan, Li Tianjie, Ji Weizhi, Yu Yang, Tan Tao
Yunnan Key Laboratory of Primate Biomedical Research; Institute of Primate Translational Medicine, Kunming University of Science and Technology, Kunming, Yunnan, China National Engineering Research Center of Biomedicine and Animal Science, Kunming, Yunnan, China.
Center of Reproductive Medicine, Department of Obstetrics and Gynecology, Peking University Third Hospital, Beijing, China.
Biol Reprod. 2015 Dec;93(6):144. doi: 10.1095/biolreprod.115.132019. Epub 2015 Nov 11.
Trophoblast stem cells differentiate into different trophoblast cell populations that are indispensable for successful pregnancy through interactions with the maternal uterine decidua. Rho GTPases play an important role in the regulation of trophoblast stem cell (TSC) self-renewal and differentiation; however, the role of Rho GDP-dissociation inhibitors (Rho GDIs) remains unclear. Here we report that overexpression of Rho GDIalpha resulted in rapid apoptosis of TSCs, while its knockdown promoted proliferation. Moreover, Rho GDIalpha knockdown also enhanced TSC invasion. Collectively, these results establish a potential mechanism whereby TSCs can balance growth and apoptosis, and thus ensure normal fetal development.
滋养层干细胞通过与母体子宫蜕膜相互作用，分化为不同的滋养层细胞群体，这些细胞群体对于成功妊娠不可或缺。Rho GTP酶在滋养层干细胞（TSC）自我更新和分化的调节中起重要作用；然而，Rho GDP解离抑制剂（Rho GDIs）的作用仍不清楚。在此我们报告，Rho GDIα的过表达导致TSC迅速凋亡，而其敲低则促进增殖。此外，Rho GDIα敲低还增强了TSC的侵袭能力。总体而言，这些结果建立了一种潜在机制，通过该机制TSC可以平衡生长和凋亡，从而确保正常的胎儿发育。
