Foley Bryan J, Drozd Ashley M, Bollard Mary T, Laspina Denise, Podobedov Nikita, Zeniou Nicholas, Rao Anjali S, Andi Babak, Jackimowicz Rick, Sweet Robert M, McSweeney Sean, Soares Alexei S
Office of Educational Programs, Brookhaven National Laboratory, Upton, NY, USA Fairmont State University, Fairmont, WV, USA.
Office of Educational Programs, Brookhaven National Laboratory, Upton, NY, USA Long Island University, Brooklyn, NY, USA.
J Lab Autom. 2016 Feb;21(1):115-24. doi: 10.1177/2211068215616072. Epub 2015 Nov 12.
A method is described for using custom snap-on lids to protect chemicals in microtiter plates from evaporation and contamination. The lids contain apertures (diameter 1.5, 1.0, or 0.5 mm) through which the chemical building blocks can be transferred. The lid with 0.5 mm apertures was tested using a noncontact acoustic liquid handler; the 1.0 and 1.5 mm lids were tested using two tip-based liquid handlers. All of the lids reduced the rate at which solvents evaporated to room air, and greatly reduced the rate of contamination by water and oxygen from room air. In steady-state measurements, the lids reduced the rate of evaporation of methanol, 1-hexene, and water by 33% to 248%. In cycled experiments, the contamination of aqueous solvent with oxygen was reduced below detectability and the rate at which DMSO engorged atmospheric water was reduced by 81%. Our results demonstrate that the lids preserve the integrity of air-sensitive reagents during the time needed for different types of liquid handlers to perform dispensations. Controlling degradation and evaporation of chemical building blocks exposed to the atmosphere is increasingly useful as the reagent volume is reduced by advances in liquid handling technology, such as acoustic droplet ejection.
本文描述了一种使用定制卡扣式盖子来保护微孔板中的化学物质免受蒸发和污染的方法。这些盖子带有孔径为1.5毫米、1.0毫米或0.5毫米的小孔，化学构建模块可通过这些小孔进行转移。使用非接触式声学液体处理仪对孔径为0.5毫米的盖子进行了测试；使用两种基于吸头的液体处理仪对孔径为1.0毫米和1.5毫米的盖子进行了测试。所有盖子都降低了溶剂向室内空气中蒸发的速率，并大大降低了室内空气中的水和氧气造成的污染速率。在稳态测量中，这些盖子使甲醇、1-己烯和水的蒸发速率降低了33%至248%。在循环实验中，水性溶剂被氧气污染的程度降低到检测限以下，二甲基亚砜吸收大气中水分的速率降低了81%。我们的结果表明，在不同类型的液体处理仪进行分配所需的时间内，这些盖子能保持对空气敏感试剂的完整性。随着液体处理技术（如声学液滴喷射）的进步使试剂体积减小，控制暴露于大气中的化学构建模块的降解和蒸发变得越来越重要。
