1,3 - 二氨基丙烷对棒状链霉菌中头孢霉素C和克拉维酸生物合成的解离作用
Dissociation of cephamycin C and clavulanic acid biosynthesis by 1,3-diaminopropane in Streptomyces clavuligerus.
作者信息
Leite Carla A, Cavallieri André P, Baptista Amanda S, Araujo Maria L G C
机构信息
Department of Biochemistry and Technological Chemistry, Institute of Chemistry, UNESP-São Paulo State University, 14800-900 Araraquara, SP, Brazil
Department of Biochemistry and Technological Chemistry, Institute of Chemistry, UNESP-São Paulo State University, 14800-900 Araraquara, SP, Brazil.
出版信息
FEMS Microbiol Lett. 2016 Jan;363(1):fnv215. doi: 10.1093/femsle/fnv215. Epub 2015 Nov 11.
Streptomyces clavuligerus produces simultaneously cephamycin C (CephC) and clavulanic acid (CA). Adding 1,3-diaminopropane to culture medium stimulates production of beta-lactam antibiotics. However, there are no studies on the influence of this diamine on coordinated production of CephC and CA. This study indicates that 1,3-diaminopropane can dissociate CephC and CA productions. Results indicated that low diamine concentrations (below 1.25 g l(-1)) in culture medium increased CA production by 200%, but not that of CephC. Conversely, CephC production increased by 300% when 10 g l(-1) 1,3-diaminopropane was added to culture medium. Addition of just L-lysine (18.3 g l(-1)) to culture medium increased both biocompounds. On the other hand, while L-lysine plus 7.5 g l(-1) 1,3-diaminopropane increased volumetric production of CephC by 1100%, its impact on CA production was insignificant. The combined results suggest that extracellular concentration of 1,3-diaminopropane may trigger the dissociation of CephC and CA biosynthesis in S. clavuligerus.
棒状链霉菌同时产生头孢霉素C（CephC）和克拉维酸（CA）。向培养基中添加1,3 - 二氨基丙烷可刺激β - 内酰胺抗生素的产生。然而，关于这种二胺对CephC和CA协同产生的影响尚无研究。本研究表明，1,3 - 二氨基丙烷可使CephC和CA的产生解离。结果表明，培养基中低浓度的二胺（低于1.25 g l⁻¹）可使CA产量增加200%，但对CephC产量无此效果。相反，当向培养基中添加10 g l⁻¹的1,3 - 二氨基丙烷时，CephC产量增加了300%。仅向培养基中添加L - 赖氨酸（18.3 g l⁻¹）可使这两种生物化合物的产量均增加。另一方面，虽然L - 赖氨酸加7.5 g l⁻¹的1,3 - 二氨基丙烷使CephC的体积产量增加了1100%，但其对CA产量的影响不显著。综合结果表明，1,3 - 二氨基丙烷的胞外浓度可能引发棒状链霉菌中CephC和CA生物合成的解离。
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