Zhang Tingting, Wang Qilin, Ye Liu, Batstone Damien, Yuan Zhiguo
Advanced Water Management Centre (AWMC), The University of Queensland, QLD 4072, Australia.
School of Chemical Engineering, The University of Queensland, QLD 4072, Australia.
Sci Rep. 2015 Nov 13;5:16631. doi: 10.1038/srep16631.
This study presents a novel pre-treatment strategy using combined free nitrous acid (FNA i.e. HNO2) and hydrogen peroxide (H2O2) to enhance methane production from WAS, with the mechanisms investigated bio-molecularly. WAS from a full-scale plant was treated with FNA alone (1.54 mg N/L), H2O2 alone (10-80 mg/g TS), and their combinations followed by biochemical methane potential tests. Combined FNA and H2O2 pre-treatment substantially enhanced methane potential of WAS by 59-83%, compared to 13-23% and 56% with H2O2 pre-treatment alone and FNA pre-treatment alone respectively. Model-based analysis indicated the increased methane potential was mainly associated with up to 163% increase in rapidly biodegradable fraction with combined pre-treatment. The molecular weight distribution and chemical structure analyses revealed the breakdown of soluble macromolecules with the combined pre-treatment caused by the deamination and oxidation of the typical functional groups in proteins, polysaccharides and phosphodiesters. These changes likely improved the biodegradability of WAS.
本研究提出了一种新型预处理策略，即使用游离亚硝酸（FNA，即HNO₂）和过氧化氢（H₂O₂）的组合来提高剩余活性污泥（WAS）的甲烷产量，并从生物分子层面研究了其作用机制。取自一座全尺寸工厂的WAS分别用单独的FNA（1.54 mg N/L）、单独的H₂O₂（10 - 80 mg/g TS）及其组合进行处理，随后进行生化甲烷潜力测试。与单独使用H₂O₂预处理（提高13 - 23%）和单独使用FNA预处理（提高56%）相比，FNA和H₂O₂联合预处理使WAS的甲烷潜力大幅提高了59 - 83%。基于模型的分析表明，联合预处理使甲烷潜力增加主要与快速生物可降解部分增加高达163%有关。分子量分布和化学结构分析表明，联合预处理导致可溶性大分子分解，这是由蛋白质、多糖和磷酸二酯中典型官能团的脱氨和氧化引起的。这些变化可能提高了WAS的生物降解性。
