a Sección Departamental de Química Analítica , Facultad de Farmacia, Universidad Complutense de Madrid , Madrid , Spain.
b Departamento de Química Física y Analítica , Universidad de Oviedo , Oviedo , Spain.
Crit Rev Food Sci Nutr. 2017 Sep 2;57(13):2758-2774. doi: 10.1080/10408398.2015.1067597.
The main goal of food safety assessment is to provide reliable information on the identity and composition of food and reduce the presence of harmful components. Nowadays, there are many countries where rather than the presence of pathogens, common public concerns are focused on the presence of hidden allergens, fraudulent practices, and genetic modifications in food. Accordingly, food regulations attempt to offer a high level of protection and to guarantee transparent information to the consumers. The availability of analytical methods is essential to comply these requirements. Protein-based strategies are usually employed for this purpose, but present some limitations. Because DNA is a more stable molecule, present in most tissues, and can be amplified, there has been an increasing interest in developing DNA-based approaches (polymerase chain reaction, microarrays, and genosensors). In this regard, electrochemical genosensors may play a major role in fulfilling the needs of food industry, such as reliable, portable, and affordable devices. This work reviews the achievements of this technology applied to allergen detection, species identification, and genetically modified organisms testing. We summarized the legislative framework, current design strategies in sensor development, their analytical characteristics, and future prospects.
食品安全评估的主要目标是提供有关食品身份和组成的可靠信息,并减少有害成分的存在。如今,许多国家关注的不是病原体的存在,而是常见的公众关注的隐藏过敏原、欺诈行为和食品中的基因改造。因此,食品法规试图提供高水平的保护,并向消费者保证透明的信息。分析方法的可用性对于满足这些要求至关重要。为此,通常采用基于蛋白质的策略,但存在一些局限性。由于 DNA 是一种更稳定的分子,存在于大多数组织中,并可以扩增,因此人们越来越有兴趣开发基于 DNA 的方法(聚合酶链反应、微阵列和基因传感器)。在这方面,电化学基因传感器可能在满足食品工业的需求方面发挥重要作用,例如可靠、便携和经济实惠的设备。这项工作综述了该技术在过敏原检测、物种鉴定和转基因生物检测方面的应用成果。我们总结了立法框架、传感器开发中的当前设计策略、它们的分析特性以及未来的前景。