Menendez-Helman Renata Julia, Sanjurjo Claudia, Miranda Patricia Vivian
Instituto de Biología y Medicina Experimental - CONICET. Buenos Aires, Argentina. ; IQUIBICEN (FCEyN, UBA - CONICET). Buenos Aires, Argentina.
Fertilab Buenos Aires, Argentina.
Iran J Reprod Med. 2015 Sep;13(9):541-8.
Glycosidases profusion in male reproductive fluids suggests a possible relationship with sperm function. Although Hexosaminidase (Hex) is the most active glycosidase in epididymal fluid and seminal plasma, as well as in spermatozoa, Glucosidase is considered a marker for epididymal function and azoospermia.
The aim of this study was to determine Hex activity in seminal plasma from patients with normal and abnormal spermograms and analyze its correlation with seminal parameters.
In this cross sectional study, seminal plasma from azoospermic, asthenozoospermic, teratozoospermic, and normozoospermic patients was analyzed for the activity of: total Hex, HexA isoform, and glucosidase. Besides, hexosamine levels were determined, and the amount of Hex was quantified by immunoblot with a specific antibody. Correlation of Hex activity with seminal parameters was also analyzed.
Hex activity, like glucosidase, was significantly reduced in azoospermic samples (44, 49, and 60% reduction for total Hex, HexA and glucosidase, respectively). A reduced amount of Hex in azoospermic samples was confirmed by western immunoblot. Hex activity was negatively correlated with round cells in azoospermic samples and positively correlated with motility in asthenozoospermic ones.
The results suggested that Hex activity was reduced in azoospermic samples and this was due to a lower amount of enzyme. The correlation to seminal parameters related to particular pathologies suggests a possible relationship of Hex with fertilizing capacity.
糖苷酶在男性生殖液中大量存在，提示其可能与精子功能有关。尽管己糖胺酶（Hex）是附睾液、精浆以及精子中最活跃的糖苷酶，但葡糖苷酶被认为是附睾功能和无精子症的标志物。
本研究旨在测定精子图谱正常和异常患者精浆中的Hex活性，并分析其与精液参数的相关性。
在这项横断面研究中，分析了无精子症、弱精子症、畸形精子症和正常精子症患者精浆中的总Hex、HexA同工型和葡糖苷酶的活性。此外，还测定了己糖胺水平，并用特异性抗体通过免疫印迹法定量Hex的含量。同时分析了Hex活性与精液参数的相关性。
与葡糖苷酶一样，无精子症样本中的Hex活性显著降低（总Hex、HexA和葡糖苷酶分别降低44%、49%和60%）。免疫印迹法证实无精子症样本中Hex的含量减少。Hex活性在无精子症样本中与圆形细胞呈负相关，在弱精子症样本中与活力呈正相关。
结果表明，无精子症样本中Hex活性降低，这是由于酶含量较低所致。与特定病理相关的精液参数的相关性表明Hex与受精能力可能存在关联。
