School of Urban and Environmental Engineering, Ulsan National Institute of Science and Technology (UNIST) , 50 UNIST-gil, Ulju-gun, Ulsan, 689-798, Republic of Korea.
Power Engineering Consulting Joint Stock Company 2 (PECC2) , 32 Ngo Thoi Nhiem str., Ward 7, District 3, Ho Chi Minh City, Vietnam.
Environ Sci Technol. 2015 Dec 15;49(24):14416-23. doi: 10.1021/acs.est.5b04310. Epub 2015 Nov 24.
The inactivation of Escherichia coli and MS2 coliphage by Cu(II) is found to be significantly enhanced in the presence of hydroxylamine (HA). The addition of a small amount of HA (i.e., 5-20 μM) increased the inactivation efficacies of E. coli and MS2 coliphage by 5- to 100-fold, depending on the conditions. Dual effects were anticipated to enhance the biocidal activity of Cu(II) by the addition of HA, viz. (i) the accelerated reduction of Cu(II) into Cu(I) (a stronger biocide) and (ii) the production of reactive oxidants from the reaction of Cu(I) with dissolved oxygen (evidenced by the oxidative transformation of methanol into formaldehyde). Deaeration enhanced the inactivation of E. coli but slightly decreased the inactivation efficacy of MS2 coliphage. The addition of 10 μM hydrogen peroxide (H2O2) greatly enhanced the MS2 inactivation, whereas the same concentration of H2O2 did not significantly affect the inactivation efficacy of E. coli Observations collectively indicate that different biocidal actions lead to the inactivation of E. coli and MS2 coliphage. The toxicity of Cu(I) is dominantly responsible for the E. coli inactivation. However, for the MS2 coliphage inactivation, the oxidative damage induced by reactive oxidants is as important as the effect of Cu(I).
羟胺（HA）的存在显著增强了铜（II）对大肠杆菌和 MS2 噬菌体的灭活作用。少量 HA（即 5-20 μM）的添加可使大肠杆菌和 MS2 噬菌体的灭活效率提高 5-100 倍，具体取决于条件。预计 HA 的添加会通过两种效应来增强 Cu(II) 的杀菌活性，即：(i) 加速将 Cu(II)还原为 Cu(I)（一种更强的杀菌剂）和 (ii) Cu(I)与溶解氧反应生成活性氧化剂（通过甲醇氧化转化为甲醛来证明）。脱氧会增强大肠杆菌的灭活，但会略微降低 MS2 噬菌体的灭活效果。添加 10 μM 过氧化氢（H2O2）会大大增强 MS2 的灭活效果，而相同浓度的 H2O2 对大肠杆菌的灭活效果没有显著影响。这些观察结果表明，不同的杀菌作用导致了大肠杆菌和 MS2 噬菌体的灭活。Cu(I) 的毒性是导致大肠杆菌灭活的主要原因。然而，对于 MS2 噬菌体的灭活，活性氧化剂引起的氧化损伤与 Cu(I)的作用同样重要。
