Wilkison W O, Sandgren E P, Palmiter R D, Brinster R L, Bell R M
Department of Biochemistry, Duke University Medical Center, Durham, NC 27710.
Oncogene. 1989 May;4(5):625-8.
Expression of the activated Harvey-ras (H-ras) oncogene in cultured cells is associated with an elevated steady-state concentration of 1,2-diacylglycerol (DG), an intracellular second messenger capable of promoting cell division. To explore the biochemistry of ras expression in vivo, we measured DG in ras-transformed neonatal liver and pancreas of transgenic mice. DG was elevated over 2-fold in these tissues compared to controls, but was not elevated in transgenic neonatal liver expressing normal H-ras, the nuclear oncogene myc, or the Simian Virus 40 T-antigens. DG was also not elevated in ras-induced lung adenomas in transgenic mice. These findings demonstrate an association between activated ras expression and DG concentration in neonatal tissue, but suggest that marked elevation of DG is not necessary for the development of ras-induced tumors in lung.
在培养细胞中，活化的哈维 - 鼠肉瘤病毒癌基因（H-ras）的表达与1,2 - 二酰甘油（DG）的稳态浓度升高有关，DG是一种能够促进细胞分裂的细胞内第二信使。为了探索体内ras表达的生物化学机制，我们测量了转基因小鼠ras转化的新生肝脏和胰腺中的DG。与对照组相比，这些组织中的DG升高了2倍以上，但在表达正常H-ras、核癌基因myc或猿猴病毒40 T抗原的转基因新生肝脏中，DG并未升高。在转基因小鼠的ras诱导的肺腺瘤中，DG也没有升高。这些发现证明了新生组织中活化的ras表达与DG浓度之间的关联，但表明DG的显著升高对于肺部ras诱导的肿瘤发生并非必要。
