Miao Qing, Wang Jing, Xu Wei, Guan Hui, Wang Qun, Liu Xiao-Ying, Huang Hui-Jie, Ren Yi-Xin, Wang Yan, Liu Yong-Ge, Li Zhen, Xiang Li
Allergy Department, Beijing Children's Hospital, Capital Medical University, 100045 Beijing, China.
Zhongguo Dang Dai Er Ke Za Zhi. 2015 Nov;17(11):1210-6.
To compare the difference in the effects of subcutaneous immunotherapy (SCIT) and sublingual immunotherapy (SLIT) on immunological responses in children with asthma.
A total of 86 children with asthma caused by dust mites were enrolled and divided into a SLIT group (n=29), a SCIT group (n=13), a group receiving complete SCIT course (complete SCIT group; n=14), and a group receiving conventional medication (control group, n=30). Peripheral blood mononuclear cells were isolated and stimulated with house dust mite extract for 48 hours in vitro, and the percentage of regulatory T cells (Treg%) in CD4+ T cells was measured by flow cytometry. Analysis of variance with repeated measures was applied to compare the changes in humoral immunological indices and therapeutic effects in the SCIT and SLIT groups before treatment and after 6 and 12 months of treatment.
Before antigenic stimulation, Treg% in CD4(+) T cells in the SCIT group was significantly higher than that in the SLIT and control groups; after antigenic stimulation was given, Treg% in the four groups decreased significantly. After 6 and 12 months of immunotherapy, the SCIT group had significant changes in serum sIgE and sIgG4 levels, while the SLIT group only showed a significant change in serum sIgE level.
Temporal difference exists in different immunotherapies to cause immunological responses in children with asthma, and immunological responses induced by SCIT may occur earlier.
比较皮下免疫疗法（SCIT）和舌下免疫疗法（SLIT）对哮喘患儿免疫反应的影响差异。
共纳入86例因尘螨引起哮喘的患儿，分为SLIT组（n = 29）、SCIT组（n = 13）、完成SCIT疗程组（完整SCIT组；n = 14）和接受传统药物治疗组（对照组，n = 30）。分离外周血单个核细胞，体外用人尘螨提取物刺激48小时，采用流式细胞术检测CD4+T细胞中调节性T细胞百分比（Treg%）。采用重复测量方差分析比较SCIT组和SLIT组治疗前、治疗6个月和12个月后体液免疫指标变化及治疗效果。
抗原刺激前，SCIT组CD4(+)T细胞中的Treg%显著高于SLIT组和对照组；给予抗原刺激后，四组的Treg%均显著降低。免疫治疗6个月和12个月后，SCIT组血清sIgE和sIgG4水平有显著变化，而SLIT组仅血清sIgE水平有显著变化。
不同免疫疗法在引起哮喘患儿免疫反应方面存在时间差异，SCIT诱导的免疫反应可能更早发生。
