Luan Chengxin, Yang Zixue, Chen Baoan
Department of Hematology and Oncology (Key Department of Jiangsu Medicine), Zhongda Hospital, School of Medicine, Southeast University, Dingjiaqiao 87, Gulou District, Nanjing, 210009, Jiangsu Province, People's Republic of China.
School of Biological Science and Medical Engineering, Southeast University, Nanjing, 210096, China.
J Fluoresc. 2016 May;26(3):1131-9. doi: 10.1007/s10895-016-1806-3. Epub 2016 Apr 11.
For analysis of biomacromolecules, a sensitive, specified and reliable method is indispensable. Fluorescent dyes or fluorophores have been widely used as mediums to obtain readout signals in various assays or bioimaging because of their versatilities such as biocompatibility. Those fluorescent dyes based techniques manipulate many molecular interactions for analysis of biomacromolecules including antibody-protein interaction, base complementation, glycan-lectin interaction, etc. The strategies to manipulate those molecular interactions are various and always updating due to the development of biotechnological tools and instruments. In this minireview, we summarize the state of the art of signal improvement techniques for fluorescence detection of biomacromolecules especially proteins and nucleic acids. We focus on the principle and mechanism of those techniques for fluorescence detection of biomacromolecules. We also discuss the future trend of the techniques for fluorescence detection of biomacromolecules.
对于生物大分子的分析而言，一种灵敏、特异且可靠的方法必不可少。荧光染料或荧光团因其具有生物相容性等多种特性，已被广泛用作在各种检测或生物成像中获取读出信号的介质。那些基于荧光染料的技术利用多种分子相互作用来分析生物大分子，包括抗体 - 蛋白质相互作用、碱基互补、聚糖 - 凝集素相互作用等。由于生物技术工具和仪器的发展，操纵这些分子相互作用的策略多种多样且不断更新。在这篇小型综述中，我们总结了生物大分子尤其是蛋白质和核酸荧光检测信号改进技术的现状。我们重点关注这些生物大分子荧光检测技术的原理和机制。我们还讨论了生物大分子荧光检测技术的未来趋势。
