Lu Ye, Ning Huijie, Jiang Xin, Yang Rui, Song Dianwen, Yuan Hongbin
Department of Anaesthesiology, Changzheng Hospital, Second Military Medical University, Shanghai, P. R. China.
Department of Bone Tumor Surgery, Changzheng Hospital, Second Military Medical University, Shanghai, P. R. China.
Biomed Chromatogr. 2017 Jul;30(7):1052-1058. doi: 10.1002/bmc.3649. Epub 2015 Nov 17.
Postoperative fatigue syndrome (POFS) is a common clinical complication followed by almost every major abdominal surgery. There is not a full explanation to the etiology of POFS, especially its central mechanism. Carthamus tinctorius L is a classic traditional Chinese medicine (TCM) which could exert the anti-fatigue effect on POFS. However, its mechanism is still lacking. Here, ultra-high performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry (UHPLC-Q-TOFMS) based metabonomic approach was used to characterize hippocampal metabolic fluctuations of POFS in a rat model induced by partial hepatectomy (PHx), and to evaluate the anti-fatigue effect of Carthamus tinctorius L extract (CTLE). With partial least-squares discriminant analysis for classification and selection of biomarkers, fifteen hippocampal metabolites related to POFS were identified, primarily involving alanine, aspartate and glutamate metabolism, valine, leucine and isoleucine degradation, purine metabolism, phenylalanine metabolism, tryptophan metabolism, phospholipid metabolism and fatty acid metabolism. With these altered metabolic pathways as possible drug targets, we systematically analyzed the protective effect of CTLE, which showed that CTLE could provide anti-fatigue effect on POFS through partially regulating the perturbed metabolic pathways. This study indicated that UHPLC-Q-TOFMS-based metabolomics provided a powerful tool to reveal hippocampal metabolic fluctuations of POFS and study the mechanism of TCM. This article is protected by copyright. All rights reserved.
术后疲劳综合征（POFS）是几乎每例腹部大手术后常见的临床并发症。目前对POFS的病因，尤其是其中心机制尚无完整解释。红花是一种经典的传统中药，对POFS具有抗疲劳作用。然而，其作用机制仍不明确。在此，采用基于超高效液相色谱-四极杆飞行时间质谱（UHPLC-Q-TOFMS）的代谢组学方法，对部分肝切除（PHx）诱导的大鼠POFS模型海马代谢波动进行表征，并评估红花提取物（CTLE）的抗疲劳作用。通过偏最小二乘判别分析进行分类和生物标志物筛选，鉴定出15种与POFS相关的海马代谢物，主要涉及丙氨酸、天冬氨酸和谷氨酸代谢、缬氨酸、亮氨酸和异亮氨酸降解、嘌呤代谢、苯丙氨酸代谢、色氨酸代谢、磷脂代谢和脂肪酸代谢。以这些改变的代谢途径作为可能的药物靶点，系统分析了CTLE的保护作用，结果表明CTLE可通过部分调节紊乱的代谢途径对POFS产生抗疲劳作用。本研究表明，基于UHPLC-Q-TOFMS的代谢组学为揭示POFS的海马代谢波动及研究中药作用机制提供了有力工具。本文受版权保护。保留所有权利。
