Gaiwala Sharma Sujata S, Kishore Vimal, Raghunand Tirumalai R
CSIR-Centre for Cellular and Molecular Biology, Uppal Road, Hyderabad 500007, India.
Res Microbiol. 2016 Feb-Mar;167(2):142-8. doi: 10.1016/j.resmic.2015.10.008. Epub 2015 Nov 11.
The major virulence trait of Mycobacterium tuberculosis is its ability to enter a latent state in the face of robust host immunity. Clues to the molecular basis of latency can emerge from understanding the mechanism of cell division, beginning with identification of proteins involved in this process. Using complementation of Escherichia coli mutants, we functionally annotated M. tuberculosis and Mycobacterium smegmatis homologs of divisome proteins FtsW and AmiC. Our results demonstrate that E. coli can be used as a surrogate model to discover mycobacterial cell division genes, and should prove invaluable in delineating the mechanisms of this fundamental process in mycobacteria.
结核分枝杆菌的主要毒力特征是其在强大的宿主免疫面前进入潜伏状态的能力。从了解细胞分裂机制入手，通过鉴定参与这一过程的蛋白质，可以找到潜伏分子基础的线索。利用大肠杆菌突变体的互补作用，我们对分枝杆菌属的细胞分裂蛋白FtsW和AmiC的结核分枝杆菌和耻垢分枝杆菌同源物进行了功能注释。我们的结果表明，大肠杆菌可以作为一种替代模型来发现分枝杆菌的细胞分裂基因，并且在阐明分枝杆菌这一基本过程的机制方面应该具有巨大价值。
