噪声应激对雄性大鼠生育能力的影响以及维生素C和E对其潜在影响的保护作用。

Effect of noise stress on male rat fertility, and the protective effect of vitamins C and E on its potential effect.

作者信息

Fathollahi Ali, Jasemi Majid, Saki Ghasem

机构信息

Emam Khomeini Hospital, Ahwaz Joundi Shapour University of Medical Sciences, Ahwaz, Iran.

Urology Ward, Golestan Hospital, Ahwaz Joundi Shapour University of Medical Sciences, Ahwaz, Iran.

出版信息

Arab J Urol. 2013 Mar;11(1):101-5. doi: 10.1016/j.aju.2012.12.002. Epub 2013 Jan 23.

DOI:10.1016/j.aju.2012.12.002

PMID:26579254

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4461010/

Abstract

OBJECTIVE

To evaluate the effect of noise on the fertility of male rats, and to assess the effect of vitamins C and E on its potential effect.

MATERIALS AND METHODS

Forty adult male rats were randomly divided into five equal groups. Group 1 (control) was not exposed to noise. Groups 2-5 were exposed to noise of 90-130 dB and 300-350 Hz from 19.00 to 07.00 h every day for 50 days; group 2 received vitamin C and group 3 received vitamin E. Group 4 received vitamins C and E concomitantly and group 5 received no vitamins. After 50 days, the serum levels of follicle-stimulating hormone (FSH), luteinising hormone (LH) and testosterone were measured. Each rat was then left for 1 week with three female rats, for mating. Pregnant females were killed humanely after 19 days of pregnancy and evaluated for the presence and number of viable, dead and absorbed fetuses.

RESULTS

The mean serum FSH level was statistically significantly different between the control and groups 2 (P < 0.05) and 5 (P < 0.001). The mean serum LH level differed significantly between the control and groups 2 (P = 0.05), 3 (P < 0.05) and 5 (P < 0.001). The mean serum testosterone level was significantly different between the control and group 5 (P < 0.001). Serum FSH, LH and testosterone levels in group 5 were significantly different from all the others (P < 0.001). The pregnancy rates in females mated with groups 1 and 5 were statistically different (P < 0.05). Comparing groups 1-4, there was no difference in the occurrence of abnormal pregnancy (P > 0.05), but group 5 values were significantly different from the others (P < 0.001).

CONCLUSION

These data strongly suggest that noise stress has a significant effect on the fertility of male rats.

摘要

目的

评估噪声对雄性大鼠生育能力的影响，并评估维生素C和维生素E对其潜在影响的作用。

材料与方法

40只成年雄性大鼠随机分为五组，每组数量相等。第1组（对照组）未暴露于噪声环境。第2 - 5组每天19:00至07:00暴露于90 - 130分贝、300 - 350赫兹的噪声环境中，持续50天；第2组给予维生素C，第3组给予维生素E。第4组同时给予维生素C和维生素E，第5组不给予维生素。50天后，检测血清促卵泡生成素（FSH）、促黄体生成素（LH）和睾酮水平。然后将每只雄性大鼠与三只雌性大鼠共同饲养1周以进行交配。怀孕雌性大鼠在妊娠19天后进行人道处死，并评估存活、死亡和吸收胎儿的存在情况及数量。

结果

对照组与第2组（P < 0.05）和第5组（P < 0.001）之间的血清FSH平均水平在统计学上有显著差异。对照组与第2组（P = 0.05）、第3组（P < 0.05）和第5组（P < 0.001）之间的血清LH平均水平有显著差异。对照组与第5组之间的血清睾酮平均水平有显著差异（P < 0.001）。第5组的血清FSH、LH和睾酮水平与其他所有组均有显著差异（P < 0.001）。与第1组和第5组雄性大鼠交配的雌性大鼠的妊娠率在统计学上有差异（P < 0.05）。比较第1 - 4组，异常妊娠的发生率无差异（P > 0.05），但第5组的值与其他组有显著差异（P < 0.001）。