原位荧光显微镜观察期间活细胞的电子显微镜检查
Electron Microscopy of Living Cells During in Situ Fluorescence Microscopy.
作者信息
Liv Nalan, van Oosten Slingeland Daan S B, Baudoin Jean-Pierre, Kruit Pieter, Piston David W, Hoogenboom Jacob P
机构信息
Department of Imaging Physics, Delft University of Technology , Lorentzweg 1, 2628 CJ Delft, The Netherlands.
Department of Molecular Physiology and Biophysics, Vanderbilt University , 747 Light Hall, Nashville, Tennessee 37232-0615, United States.
出版信息
ACS Nano. 2016 Jan 26;10(1):265-73. doi: 10.1021/acsnano.5b03970. Epub 2015 Dec 8.
Abstract
We present an approach toward dynamic nanoimaging: live fluorescence of cells encapsulated in a bionanoreactor is complemented with in situ scanning electron microscopy (SEM) on an integrated microscope. This allows us to take SEM snapshots on-demand, that is, at a specific location in time, at a desired region of interest, guided by the dynamic fluorescence imaging. We show that this approach enables direct visualization, with EM resolution, of the distribution of bioconjugated quantum dots on cellular extensions during uptake and internalization.
摘要
我们提出了一种动态纳米成像方法
封装在生物纳米反应器中的细胞的实时荧光通过集成显微镜上的原位扫描电子显微镜（SEM）得到补充。这使我们能够按需拍摄SEM快照，即在动态荧光成像的引导下，在特定的时间点、在所需的感兴趣区域拍摄。我们表明，这种方法能够以电子显微镜分辨率直接观察生物共轭量子点在摄取和内化过程中在细胞延伸部分上的分布。
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