对患有急性细支气管炎或复发性喘息的四岁以下儿童进行腺病毒C种检测。
Adenovirus species C detection in children under four years of age with acute bronchiolitis or recurrent wheezing.
作者信息
Tórtora Rosângela Prendin, Guimarães Maria Angélica Arpon Marandino, de Souza Leandro Magalhães, Santos Isabela Arruda, Varella Rafael Brandão, de Fátima Pombo March Maria, da Cunha Antonio Jose Ledo Alves, Sant' Anna Clemax Couto
机构信息
Laboratório do Nucleo de Apoio a Criança e ao Adolescente do Instituto de Puericultura e Pediatria Martagão Gesteira, UFRJ, Brazil; Laboratório de Virologia, Hospital Universitário Clementino Fraga Filho, UFRJ, Brazil.
Laboratório do Nucleo de Apoio a Criança e ao Adolescente do Instituto de Puericultura e Pediatria Martagão Gesteira, UFRJ, Brazil; Laboratório de Virologia, Hospital Universitário Clementino Fraga Filho, UFRJ, Brazil; Depto de Medicina Preventiva, Faculdade de Medicina, UFRJ, Brazil; Programa de Pós graduação em Doenças Infecciosas, Faculdade de Medicina, UFRJ, Brazil.
出版信息
J Clin Virol. 2015 Dec;73:77-80. doi: 10.1016/j.jcv.2015.11.007. Epub 2015 Nov 5.
BACKGROUND
Lower respiratory tract viral infection is an important cause of morbidity and mortality in children worldwide. Among viral etiological agents the human Adenovirus (AdV) has been associated to mild or severe respiratory tract infection.
OBJECTIVE
To detect the presence of human Adenovirus (AdV) in children with acute bronchiolitis or recurrent wheezing, describing their clinical features and determining Adenovirus species and AdV association to Respiratory Syncytial Virus (RSV), Human Metapneumovirus (MPV) and Parainfluenza virus (PIV).
STUDY DESIGN
A total of 155 children bellow 48 months of age with acute bronchiolitis or recurrent wheezing were investigated for the presence of AdV, RSV, MPV and PIV in nasopharyngeal aspirate, by real-time PCR method.
RESULTS
AdV, predominantly of species C, has been detected as the unique pathogen (AdVi) or in association to other pathogens (AdVa.), in 39/155 samples. Crackles were more frequent in children with AdV. RSVi was detected predominantly in children with acute bronchiolitis while AdVi and AdVa were detected more frequently in patients with recurrent wheezing.
CONCLUSION
A small outbreak of AdV species C was observed in 2012 and 2013. AdV was detected more frequently in children with recurrent wheezing while RSVi was more frequent in infants with acute bronchiolitis.
背景
下呼吸道病毒感染是全球儿童发病和死亡的重要原因。在病毒病原体中，人类腺病毒（AdV）与轻度或重度呼吸道感染有关。
目的
检测急性细支气管炎或反复喘息儿童中人类腺病毒（AdV）的存在，描述其临床特征，确定腺病毒种类以及AdV与呼吸道合胞病毒（RSV）、人偏肺病毒（MPV）和副流感病毒（PIV）的关联。
研究设计
采用实时聚合酶链反应方法，对155名48个月以下患有急性细支气管炎或反复喘息的儿童的鼻咽抽吸物进行检测，以确定是否存在AdV、RSV、MPV和PIV。
结果
在155份样本中，有39份检测到AdV，主要为C种，它可作为唯一病原体（AdVi）或与其他病原体合并存在（AdVa）。AdV感染的儿童中，细湿啰音更为常见。RSVi主要在急性细支气管炎儿童中检测到，而AdVi和AdVa在反复喘息患者中更常见。
结论
2012年和2013年观察到C种AdV的小规模暴发。反复喘息儿童中AdV检测更为频繁，而急性细支气管炎婴儿中RSVi更为常见。
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