Kai Song, Cheng-Wen Lu, Yi-Nan Ding, Tian Luan, Guang-Ye Wang, Jing-Mei Lu, Li-Quan Guo
School of Life Science, Changchun Normal University, Changchun 130032, China.
School and Life Science, Northeast Normal University, Changchun 130024, China.
Spectrochim Acta A Mol Biomol Spectrosc. 2016 Feb 15;155:81-7. doi: 10.1016/j.saa.2015.11.009. Epub 2015 Nov 7.
In this paper, we reported an optical sensing composite for cysteine detection. A chemosensor derived from rhodamine 6G was synthesized and characterized. To minimize its photobleaching, up-conversion nanocrystals β-NaYF4:Yb(3+)/Er(3+) were prepared and modified with α-cyclodextrin, serving as excitation host. Under 980nm laser excitation, emission of these up-conversion nanocrystals overlapped well with the absorption of our chemosensor. Energy transfer between them was analyzed and confirmed by emission decay analysis. Job's analysis suggested that the complexation equilibrium between our chemosensor and cysteine was a simple one with binding stoichiometry of 1:1. A sensing system was constructed with up-conversion nanocrystals (modified with α-cyclodextrin) and this chemosensor. Emission "turn-on" effect was observed only for cysteine but immune to other competing amino acids and thiols, showing a good selectivity.
在本文中，我们报道了一种用于检测半胱氨酸的光学传感复合材料。合成并表征了一种由罗丹明6G衍生的化学传感器。为了使其光漂白最小化，制备了上转换纳米晶体β-NaYF4:Yb(3+)/Er(3+)并用α-环糊精进行修饰，作为激发主体。在980nm激光激发下，这些上转换纳米晶体的发射与我们化学传感器的吸收良好重叠。通过发射衰减分析对它们之间的能量转移进行了分析和确认。Job分析表明，我们的化学传感器与半胱氨酸之间的络合平衡是简单的1:1结合化学计量比。构建了一个用上转换纳米晶体（用α-环糊精修饰）和这种化学传感器的传感系统。仅对半胱氨酸观察到发射“开启”效应，而对其他竞争性氨基酸和硫醇免疫，显示出良好的选择性。
