通过将iTRAQ等压标记与选择反应监测/多反应监测（SRM/MRM）相结合对组织样本进行定量分析。

Quantitative Analysis of Tissue Samples by Combining iTRAQ Isobaric Labeling with Selected/Multiple Reaction Monitoring (SRM/MRM).

作者信息

Narumi Ryohei, Tomonaga Takeshi

机构信息

Laboratory for Synthetic Biology, RIKEN Quantitative Biology Center, Osaka, Japan.

Laboratory of Proteome Research, National Institutes of Biomedical Innovation, Health and Nutrition, Osaka, Japan.

出版信息

Methods Mol Biol. 2016;1355:85-101. doi: 10.1007/978-1-4939-3049-4_6.

DOI:10.1007/978-1-4939-3049-4_6

PMID:26584920

Abstract

Mass spectrometry-based phosphoproteomics is an indispensible technique used in the discovery and quantification of phosphorylation events on proteins in biological samples. The application of this technique to tissue samples is especially useful for the discovery of biomarkers as well as biological studies. We herein describe the application of a large-scale phosphoproteome analysis and SRM/MRM-based quantitation to develop a strategy for the systematic discovery and validation of biomarkers using tissue samples.

摘要

基于质谱的磷酸化蛋白质组学是一种用于发现和定量生物样品中蛋白质磷酸化事件的不可或缺的技术。将该技术应用于组织样品对于生物标志物的发现以及生物学研究尤为有用。我们在此描述了大规模磷酸化蛋白质组分析和基于SRM/MRM的定量分析的应用，以开发一种使用组织样品系统发现和验证生物标志物的策略。