Fietz Daniela, Bergmann M, Hartmann K
Institute for Veterinary Anatomy, Histology and Embryology, Justus Liebig University Giessen, Frankfurter Straße 98, 35392, Gießen, Germany.
Methods Mol Biol. 2016;1366:189-205. doi: 10.1007/978-1-4939-3127-9_15.
In situ hybridization (ISH) is an excellent method for detecting RNA in histological sections, both to detect gene expression and to assign gene expression to a distinct cell population. Therefore, ISH may be used in basic cell biology to detect the expression of certain genes within a tissue containing various cell populations. Here, we describe the detection and cellular localization of three estrogen receptors, both isoforms of the genomic estrogen receptor (ERα and ERβ) as well as the membrane-bound G-protein-coupled estrogen receptor 1 (GPER) in the human testis.
原位杂交（ISH）是检测组织切片中RNA的一种优秀方法，既可以检测基因表达，也可以将基因表达定位到不同的细胞群体。因此，ISH可用于基础细胞生物学，以检测包含各种细胞群体的组织内某些基因的表达。在此，我们描述了人类睾丸中三种雌激素受体的检测及细胞定位，包括基因组雌激素受体的两种亚型（ERα和ERβ）以及膜结合型G蛋白偶联雌激素受体1（GPER）。
