利用卡马西平、HF183 拟杆菌和粪便指示菌检测波多黎各的人源性粪便污染。
Detection of human-derived fecal contamination in Puerto Rico using carbamazepine, HF183 Bacteroides, and fecal indicator bacteria.
机构信息
Department of Marine Sciences, University of Puerto Rico, Mayaguez Campus, P.O. Box 9013, Mayaguez, PR 00681, USA.
Department of Marine Sciences, University of Puerto Rico, Mayaguez Campus, P.O. Box 9013, Mayaguez, PR 00681, USA.
出版信息
Mar Pollut Bull. 2015 Dec 30;101(2):872-7. doi: 10.1016/j.marpolbul.2015.11.016. Epub 2015 Nov 14.
The level of fecal pollution in 17 sites in Puerto Rico was determined by Escherichia coli (E.coli) enumeration using an enzyme substrate medium and Quanti-Tray®/2000. Human fecal pollution was identified using an enzyme-linked immunosorbent assay for the detection of carbamazepine (CBZ) and quantitative polymerase chain reaction (qPCR) detection of the human Bacteroides marker, HF183. Carbamazepine was detected in 16 out of 17 sites, including Condado Lagoon, a popular recreational area. Elevated E.coli levels (>410 CFU 100 mL(-1)) were detected in 13 sites. Average CBZ concentrations ranged from 0.005 μg L(-1) to 0.482 μg L(-1) and 7 sites were positive for HF183. Higher CBZ concentrations were associated with the detection of HF183 (Mann-Whitney test; U=42.0; df=7; 1-tailed P value=0.013). This was the second study to determine surface water concentrations of CBZ in the Caribbean and the first in Puerto Rico.
对波多黎各 17 个地点的粪便污染水平进行了测定，方法是使用酶底物培养基和 Quanti-Tray®/2000 对大肠杆菌 (E.coli) 进行计数。采用酶联免疫吸附测定法检测卡马西平 (CBZ) ，并用定量聚合酶链反应 (qPCR) 检测人类双歧杆菌标记物 HF183，来鉴定人类粪便污染。在 17 个地点中，有 16 个地点检测到了卡马西平，其中包括一个受欢迎的娱乐区 Condado Lagoon。在 13 个地点检测到大肠杆菌水平升高 (>410 CFU 100 mL(-1))。CBZ 的平均浓度范围为 0.005 μg L(-1) 至 0.482 μg L(-1)，有 7 个地点 HF183 呈阳性。HF183 的检测与 CBZ 浓度较高有关 (Mann-Whitney 检验；U=42.0；df=7；单侧 P 值=0.013)。这是加勒比地区第二次测定地表水中 CBZ 浓度的研究，也是波多黎各的第一次。
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