Ghanbarzadeh Corehtash Zahra, Khorshidi Ahmad, Firoozeh Farzaneh, Akbari Hosein, Mahmoudi Aznaveh Azam
Department of Microbiology and Immunology, Faculty of Medicine, Kashan University of Medical Sciences, Kashan, IR Iran.
Biostatistics Department, Truma Research center, Kashan University of Medical Sciences, Kashan, IR Iran.
Jundishapur J Microbiol. 2015 Oct 21;8(10):e22345. doi: 10.5812/jjm.22345. eCollection 2015 Oct.
Pseudomonas aeruginosa possesses a variety of virulence factors and infections caused by multidrug-resistant P. aeruginosa (MDRPA) in burn patients are a public health problem.
The aim of this study was to determine the antibiotic resistance pattern, the biofilm formation, the prevalence of MDRPA and two virulence genes (nan1 and exoA) among P. aeruginosa isolated from burn patients.
A total of 144 isolates of P. aeruginosa were collected from burn patient at the Burn Centre of Tehran, Iran, between March 2013 and July 2013. Antibiotic susceptibility test was performed via agar disk diffusion method. The ability of producing biofilm was examined by crystal violet microtiter plate assay and the prevalence of the exoA and nan1 genes among the isolates was determined by polymerase chain reaction (PCR).
A high rate of resistance was seen against ciprofloxacin (93.7%), aztreonam (86.8%), piperacillin (85.4%), ceftazidime (82.6%), amikacin (82%) and imipenem (79.2%). In total, 93.1% of the isolates were characterized as MDRPA. Biofilm formation was seen in 92.4% of the isolates. The prevalence of the exoA and nan1 genes were 75% and 11.8% among the isolates, respectively.
The high rate of MDRPA and its ability to produce biofilm is an alarm for public health. The statistical analysis showed that biofilm production in the MDRPA isolates was significantly higher than that in the non-MDRPA isolates (P < 0.001).
铜绿假单胞菌具有多种毒力因子，烧伤患者中由多重耐药铜绿假单胞菌（MDRPA）引起的感染是一个公共卫生问题。
本研究旨在确定从烧伤患者中分离出的铜绿假单胞菌的抗生素耐药模式、生物膜形成情况、MDRPA的流行率以及两个毒力基因（nan1和exoA）。
2013年3月至2013年7月期间，从伊朗德黑兰烧伤中心的烧伤患者中总共收集了144株铜绿假单胞菌分离株。通过琼脂纸片扩散法进行抗生素敏感性试验。采用结晶紫微量滴定板法检测生物膜形成能力，并通过聚合酶链反应（PCR）确定分离株中exoA和nan1基因的流行率。
观察到对环丙沙星（93.7%）、氨曲南（86.8%）、哌拉西林（85.4%）、头孢他啶（82.6%）、阿米卡星（82%）和亚胺培南（79.2%）的高耐药率。总体而言，93.1%的分离株被鉴定为MDRPA。92.4%的分离株观察到生物膜形成。分离株中exoA和nan1基因的流行率分别为75%和11.8%。
MDRPA的高流行率及其产生生物膜的能力对公共卫生是一个警示。统计分析表明，MDRPA分离株中的生物膜产生明显高于非MDRPA分离株（P < 0.001）。
