Dunn Elyse A, Cook Gregory M, Heikal Adam
Department of Microbiology and Immunology, University of Otago, Dunedin 9054, New Zealand.
Department of Microbiology and Immunology, University of Otago, Dunedin 9054, New Zealand; Maurice Wilkins Centre for Molecular Biodiscovery, The University of Auckland, Private Bag 92019, Auckland 1042, New Zealand.
J Microbiol Methods. 2016 Jan;120:41-3. doi: 10.1016/j.mimet.2015.11.012. Epub 2015 Nov 28.
This study compared detergent-solubilised (soluble) and lipid-reconstituted (proteoliposome) protein to establish a high-throughput method for identifying membrane protein inhibitors. We identified inhibitors of the membrane-bound type II NADH dehydrogenase with lower lipophilicity and better potency, suggesting proteoliposome systems may be advantageous over detergent-solubilised systems for respiratory membrane proteins.
本研究比较了去污剂增溶（可溶性）蛋白和脂质重构（蛋白脂质体）蛋白，以建立一种高通量方法来鉴定膜蛋白抑制剂。我们鉴定出了亲脂性较低且效力更佳的膜结合型II NADH脱氢酶抑制剂，这表明对于呼吸膜蛋白而言，蛋白脂质体系统可能比去污剂增溶系统更具优势。
