Miller B M, Adler I D
Institut für Säugetiergenetik, Gesellschaft für Strahlen- und Umweltforschung, Neutherberg, FRG.
Mutagenesis. 1989 May;4(3):208-15. doi: 10.1093/mutage/4.3.208.
In the coordinated programme to study aneuploidy induction, sponsored by the Commission of the European Communities, 10 known or suspect spindle poisons (colchicine, econazole, chloralhydrate, hydroquinone, diazepam, thiabendazole, cadmium chloride, pyrimethamine, vincristine and thimerosal) were tested in mouse bone marrow cells for the induction of c-mitotic effects. Three criteria were chosen: changes of the mitotic index, induction of chromatid contraction and spreading and decrease of anaphase frequencies. Among the chemicals tested colchicine, econazole, chloralhydrate, hydroquinone and vincristine were found positive. Diazepam, thiabendazole, cadmium chloride, pyrimethamine and thimerosal revealed no induction of c-mitotic effects under the conditions tested. Mitotic block and subsequent chromosome malsegregation (non-disjunction) are related phenomena. The three criteria chosen are considered as an indicative pre-screening test for the aneuploidy inducing potency of a chemical in mitotic, but not in meiotic cells. The present experiments were also regarded as a dose-finding exercise for further in vivo studies.
在由欧洲共同体委员会赞助的研究非整倍体诱导的协调计划中，对10种已知或可疑的纺锤体毒物（秋水仙碱、益康唑、水合氯醛、对苯二酚、地西泮、噻苯达唑、氯化镉、乙胺嘧啶、长春新碱和硫柳汞）在小鼠骨髓细胞中进行了诱导c-有丝分裂效应的测试。选择了三个标准：有丝分裂指数的变化、染色单体收缩和分散的诱导以及后期频率的降低。在所测试的化学物质中，发现秋水仙碱、益康唑、水合氯醛、对苯二酚和长春新碱呈阳性。在地西泮、噻苯达唑、氯化镉、乙胺嘧啶和硫柳汞所测试的条件下未显示出诱导c-有丝分裂效应。有丝分裂阻滞和随后的染色体错误分离（不分离）是相关现象。所选择的三个标准被视为对化学物质在有丝分裂而非减数分裂细胞中诱导非整倍体能力的指示性预筛选试验。本实验也被视为进一步体内研究的剂量探索试验。
