Plyuta V A, Lipasova V A, Koksharova O A, Veselova M A, Kuznetsov A E, Khmel I A
Genetika. 2015 Aug;51(8):864-72.
To study the role of Quorum Sensing (QS) regulation in the control of the cellular processes of Burkholderia cenocepacia 370, plasmid pME6863 was transferred into its cells. The plasmid contains a heterologous gene encoding for AiiA N-acyl-homoserine lactonase, which degrades the signaling molecules of the QS system of N-acyl-homoserine lactones (AHL). An absence or reduction of AHL in the culture was revealed with the biosensors Chromobacterium violaceum CV026 and Agrobacterium tumifaciens NT1/pZLR4, respectively. The presence of the aiiA gene, which was cloned from Bacillus sp. A24 in the cells of B. cenocepacia 370, resulted in a lack of hemolytic activity, which reduced the extracellular proteolytic activity and decreased the cells' ability to migration in swarms on the surface of the agar medium. The introduction of the aiiA gene did not affect lipase activity, fatty acids synthesis, HCN synthesis, or biofilm formation. Hydrogen peroxide was shown to stimulate biofilm formation by B. cenocepacia 370 in concentrations that inhibited or weakly suppressed bacterial growth. The introduction of the aiiA gene into the cells did not eliminate this effect but it did reduce it.
为了研究群体感应（QS）调控在控制洋葱伯克霍尔德菌370细胞过程中的作用，将质粒pME6863转入其细胞中。该质粒含有一个编码AiiA N-酰基高丝氨酸内酯酶的异源基因，该酶可降解N-酰基高丝氨酸内酯（AHL）的QS系统的信号分子。分别用紫色色杆菌CV026和根癌农杆菌NT1/pZLR4生物传感器检测到培养物中AHL的缺失或减少。从芽孢杆菌属A24克隆的aiiA基因在洋葱伯克霍尔德菌370细胞中的存在导致溶血活性缺乏，这降低了细胞外蛋白水解活性，并降低了细胞在琼脂培养基表面群体迁移的能力。aiiA基因的导入不影响脂肪酶活性、脂肪酸合成、HCN合成或生物膜形成。结果表明，过氧化氢在抑制或微弱抑制细菌生长的浓度下能刺激洋葱伯克霍尔德菌370形成生物膜。将aiiA基因导入细胞并没有消除这种作用，但确实减弱了这种作用。
