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[利用特定DNA标记鉴定油菜(甘蓝型油菜L.)中FAD3基因的等位基因]

[The Use of Specific DNA Markers for the Identification of Alleles of the FAD3 Genes in Rape (Brassica napus L.)].

作者信息

Lemesh V A, Mozgova G V, Grushetskaya Z E, Sidorenko E V, Pilyuk Ya E, Bakanovskaya A V

出版信息

Genetika. 2015 Aug;51(8):895-904.

Abstract

A search was conducted for the alleles responsible for the quality of food-grade rapeseed oil in a collection of 21 samples of spring and winter oilseed rape of Belarusian and Russian breeding. We also developed A- and C-gene-specific DNA markers to assess the genomic polymorphisms of rape for FAD3 genes and selected plants with a low content of linolenic acid for use in the selection process. The development of a method for identifying FAD3 alleles, which control the level of linolenic acid in rapeseed oil, as well as of the design for new dCAPS markers, enabled the identification of plants homozygous for individual FAD3A and/or FAD3C genes in the F2-generation. These plants are currently involved in the selection process of new varieties with a reduced content of linolenic acid in rapeseed oil.

摘要

对来自白俄罗斯和俄罗斯育种的21个春播和冬播油菜籽样本进行了研究,以寻找决定食品级菜籽油品质的等位基因。我们还开发了A基因和C基因特异性DNA标记,以评估油菜FAD3基因的基因组多态性,并选择亚麻酸含量低的植株用于选育过程。开发一种鉴定控制菜籽油中亚麻酸水平的FAD3等位基因的方法以及设计新的dCAPS标记,使得能够在F2代中鉴定出单个FAD3A和/或FAD3C基因纯合的植株。这些植株目前正参与菜籽油中亚麻酸含量降低的新品种选育过程。

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