伊朗马库伊肥尾羊品种中生长激素（GH）和瘦素（LEP）基因多态性与肥尾测量值（肥尾尺寸）的关系。

The relationship of GH and LEP gene polymorphisms with fat-tail measurements (fat-tail dimensions) in fat-tailed Makooei breed of Iranian sheep.

作者信息

Hajihosseinlo Abbas, Jafari Shoja, Ajdary Marziyeh

机构信息

Genetics and Animal Breeding, Aras International Campus, University of Tehran, Jolfa, Iran.

Department of Animal Sciences, Urmia University, Urmia, Iran.

出版信息

Adv Biomed Res. 2015 Aug 31;4:172. doi: 10.4103/2277-9175.163995. eCollection 2015.

DOI:10.4103/2277-9175.163995

PMID:26605211

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4617003/

Abstract

BACKGROUND

The present study was designed to investigate the association of GH and LEP genes' single-nucleotide polymorphisms with fat-tail measurements (fat-tail dimensions) in Makooei sheep.

MATERIALS AND METHODS

DNA was extracted from whole blood samples collected from 100 sheep. Polymerase chain reaction (PCR) products were subjected to single-strand conformation polymorphism (SSCP) denaturation and polyacrylamide gel electrophoresis. Data were collected at the Makooei Sheep Breeding Station in Makoo (36°, 35'S and 48°, 22'E) of West Azerbaijan province. Climatically, this location has temperate summers and cold winters and receives a mean annual rainfall of about 400 mm. Ewes are raised in an annual breeding cycle starting in September. In general, the flock is managed under a semi-migratory system.

RESULTS

In the tested Makooei sheep population, significant statistical results were found in all traits of fat-tail measurements for GH and LEP genes. Individuals with the G4, L4 genotype of GH and LEP genes had lower tail length (rump length), fat thickness (the thick rump), and tail width (rump width) when compared to those of individuals with other genotypes (P < 0.05). In addition, the results demonstrated that individuals with the G5, L5 genotype of GH and LEP genes had superiority of tail length (rump length) and fat thickness (the thick rump) compared to those individuals with other genotypes (P < 0.05). Individuals with the G2, L2 genotype of GH and LEP genes had superiority of tail width (rump width) compared individuals with other genotypes (P < 0.05).

CONCLUSION

These results confirmed potential usefulness of GH and LEP genes in marker-assisted selection programs of sheep breeding.

摘要

背景

本研究旨在调查生长激素（GH）和瘦素（LEP）基因的单核苷酸多态性与马库伊绵羊肥尾测量值（肥尾尺寸）之间的关联。

材料与方法

从100只绵羊采集的全血样本中提取DNA。聚合酶链反应（PCR）产物进行单链构象多态性（SSCP）变性和聚丙烯酰胺凝胶电泳。数据在西阿塞拜疆省马库（南纬36°35′，东经48°22′）的马库伊绵羊育种站收集。该地区气候特点是夏季温和、冬季寒冷，年平均降雨量约400毫米。母羊于9月开始进入年度繁殖周期。总体而言，羊群采用半游牧系统管理。

结果

在受试的马库伊绵羊群体中，GH和LEP基因的所有肥尾测量性状均发现显著的统计结果。与其他基因型个体相比，GH和LEP基因的G4、L4基因型个体的尾长（臀长）、脂肪厚度（厚臀）和尾宽（臀宽）较低（P < 0.05）。此外，结果表明，GH和LEP基因的G5、L5基因型个体在尾长（臀长）和脂肪厚度（厚臀）方面优于其他基因型个体（P < 0.05）。GH和LEP基因的G2、L2基因型个体在尾宽（臀宽）方面优于其他基因型个体（P < 0.05）。