用于细胞集落分选的大面积磁性微托盘阵列
Large area magnetic micropallet arrays for cell colony sorting.
作者信息
Cox-Muranami Wesley A, Nelson Edward L, Li G P, Bachman Mark
机构信息
Biomedical Engineering, University of California, Irvine, CA, USA.
School of Medicine, Department of Medicine, School of Biological Sciences, Department of Molecular Biology and Biochemistry, University of California, Irvine, CA, USA.
出版信息
Lab Chip. 2016 Jan 7;16(1):172-81. doi: 10.1039/c5lc01131k.
A new micropallet array platform for adherent cell colony sorting has been developed. The platform consisted of thousands of square plastic pallets, 270 μm by 270 μm on each side, large enough to hold a single colony of cells. Each pallet included a magnetic core, allowing them to be collected with a magnet after being released using a microscope mounted laser system. The micropallets were patterned from 1002F epoxy resist and were fabricated on translucent, gold coated microscope slides. The gold layer was used as seed for electroplating the ferromagnetic cores within every individual pallet. The gold layer also facilitated the release of each micropallet during laser release. This array allows for individual observation, sorting and collection of isolated cell colonies for biological cell colony research. In addition to consistent release and recovery of individual colonies, we demonstrated stable biocompatibility and minimal loss in imaging quality compared to previously developed micropallet arrays.
一种用于贴壁细胞集落分选的新型微托盘阵列平台已被开发出来。该平台由数千个正方形塑料托盘组成，每个托盘边长为270μm，大到足以容纳单个细胞集落。每个托盘都包含一个磁芯，使得它们在使用安装在显微镜上的激光系统释放后能够被磁铁收集。这些微托盘由1002F环氧抗蚀剂制成图案，并制作在半透明的、镀有金的显微镜载玻片上。金层用作电镀每个单独托盘中铁磁芯的种子层。金层在激光释放过程中也有助于每个微托盘的释放。该阵列允许对分离的细胞集落进行单独观察、分选和收集，用于生物细胞集落研究。除了单个集落的一致释放和回收外，与先前开发的微托盘阵列相比，我们还证明了其具有稳定的生物相容性且成像质量损失最小。
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