School of Engineering, Department of Biomedical Engineering, University of California at Irvine, Irvine, California 92697, United States.
Langmuir. 2010 Nov 16;26(22):17703-11. doi: 10.1021/la101960v. Epub 2010 Oct 22.
We present a magnetic micropallet array and demonstrate its capacity to recover specific, individual adherent cells from large populations and deliver them for downstream single cell analysis. A ferromagnetic photopolymer was formulated, characterized, and used to fabricate magnetic micropallets, which are microscale pedestals that provide demarcated cell growth surfaces with preservation of biophysical properties including photopatternability, biocompatibility, and optical clarity. Each micropallet holds a single adherent cell in culture, and hundreds of thousands of micropallets comprise a single micropallet array. Any micropallet in the array can be recovered on demand, carrying the adhered cell with it. We used this platform to recover selectively single cells, which were subsequently analyzed using single-cell RT-qPCR.
我们提出了一种磁性微托盘阵列，并展示了它从大量细胞中特异性回收单个贴壁细胞并将其用于下游单细胞分析的能力。我们制备、表征了一种铁磁光聚合物，并利用它制造了磁性微托盘，这是一种微尺度的基座，提供了划定的细胞生长表面，保留了包括光可操作性、生物相容性和光学透明度在内的生物物理特性。每个微托盘在培养中容纳一个单个贴壁细胞，成千上万的微托盘组成一个单个微托盘阵列。该阵列中的任何微托盘都可以按需回收，带着附着的细胞一起回收。我们使用这个平台有选择地回收单个细胞，然后使用单细胞 RT-qPCR 对其进行分析。
