Bähr Ariane, Singer Anna, Hain Anika, Vasudevan Ananda Ayyappan Jaguva, Schilling Mirjam, Reh Juliane, Riess Maximilian, Panitz Sylvia, Serrano Vanessa, Schweizer Matthias, König Renate, Chanda Sumit, Häussinger Dieter, Kochs Georg, Lindemann Dirk, Münk Carsten
Clinic for Gastroenterology, Hepatology, and Infectiology, Medical Faculty, Heinrich-Heine-University, Moorenstr. 5, 40225 Düsseldorf, Germany.
Institute of Virology, University Medical Center Freiburg, Herman-Herder-Str. 1, 79104 Freiburg, Germany.
Virology. 2016 Jan 15;488:51-60. doi: 10.1016/j.virol.2015.10.034. Epub 2015 Nov 21.
Foamy viruses (FV) are retroviruses that are widely distributed in primate and non-primate animal species. We tested here FV with capsids of simian and non-simian origin for sensitivity to interferon-β (IFN-β). Our data show significant inhibition of FV by IFN-β early in infection of human HOS and THP-1 but not of HEK293T cells. The post-entry restriction of FV was not mediated by the interferon-induced MxB protein that was recently identified as a capsid-interacting restriction factor targeting Human immunodeficiency virus (HIV) before integration. Neither the ectopic expression of MxA or MxB in HEK293T cells nor the lack of MxB expression in CRISPR/CAS MxB THP-1 knockout cells impacted the infection of the tested FV. IFN-β treated THP-1 and THP-1 KO MxB cells showed the same extend of restriction to FV. Together, the data demonstrate that IFN-β inhibits FV early in infection and that MxB is not a restriction factor of FV.
泡沫病毒（FV）是逆转录病毒，广泛分布于灵长类和非灵长类动物物种中。我们在此测试了具有猿猴和非猿猴来源衣壳的FV对干扰素-β（IFN-β）的敏感性。我们的数据显示，在人HOS和THP-1细胞感染早期，IFN-β对FV有显著抑制作用，但对HEK293T细胞无此作用。FV进入后的限制作用不是由干扰素诱导的MxB蛋白介导的，MxB蛋白最近被鉴定为整合前靶向人类免疫缺陷病毒（HIV）的衣壳相互作用限制因子。在HEK293T细胞中异位表达MxA或MxB，以及CRISPR/CAS MxB THP-1基因敲除细胞中缺乏MxB表达，均不影响所测试FV的感染。用IFN-β处理的THP-1和THP-1 KO MxB细胞对FV的限制程度相同。总之，数据表明IFN-β在感染早期抑制FV，且MxB不是FV的限制因子。
