小鼠黑色素瘤细胞中的kin17蛋白
The kin17 Protein in Murine Melanoma Cells.
作者信息
Ramos Anelise C, Gaspar Vanessa P, Kelmer Sabrina M G, Sellani Tarciso A, Batista Ana G U, De Lima Neto Quirino A, Rodrigues Elaine G, Fernandez Maria A
机构信息
Departamento de Biotecnologia, Genética e Biologia Celular, Universidade Estadual de Maringá, 87020-900 Maringá, Paraná, Brazil.
Laboratório de Imunobiologia do Câncer, Departamento de Microbiologia, Imunologia e Parasitologia, Escola Paulista de Medicina, Universidade Federal de São Paulo, 04023-062 São Paulo, São Paulo, Brazil.
出版信息
Int J Mol Sci. 2015 Nov 24;16(11):27912-20. doi: 10.3390/ijms161126072.
kin17 has been described as a protein involved in the processes of DNA replication initiation, DNA recombination, and DNA repair. kin17 has been studied as a potential molecular marker of breast cancer. This work reports the detection and localization of this protein in the murine melanoma cell line B16F10-Nex2 and in two derived subclones with different metastatic potential, B16-8HR and B16-10CR. Nuclear and chromatin-associated protein fractions were analyzed, and kin17 was detected in all fractions, with an elevated concentration observed in the chromatin-associated fraction of the clone with low metastatic potential, suggesting that the kin17 expression level could be a marker of melanoma.
kin17已被描述为一种参与DNA复制起始、DNA重组和DNA修复过程的蛋白质。kin17已作为乳腺癌的潜在分子标志物进行研究。这项工作报道了该蛋白在小鼠黑色素瘤细胞系B16F10-Nex2以及两个具有不同转移潜能的衍生亚克隆B16-8HR和B16-10CR中的检测和定位。分析了核蛋白和与染色质相关的蛋白组分，在所有组分中均检测到kin17，在转移潜能低的克隆的染色质相关组分中观察到其浓度升高，这表明kin17表达水平可能是黑色素瘤的一个标志物。
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