Xin Hua-Mei, Zhu Yuemin
College of Physics & Electronics, Shandong Normal University, 250014, Shandong, China.
CREATIS, CNRS UMR 5220, Inserm U1044, INSA Lyon, University of Lyon, Bâtiment Blaise Pascal, 69621, Villeurbanne cedex, France.
Methods Mol Biol. 2016;1384:109-17. doi: 10.1007/978-1-4939-3255-9_7.
The analysis of a large number of two-dimensional gel electrophoresis (2-DE) images requires developing automatic methods. In such analyses, spot matching plays a fundamental role, in particular for the identification of proteins. We describe a simple and accurate method which allows to automatically and accurately match spots in 2-DE images. The method consists of simultaneously exploiting the distance between the spots, their intensity, and the pattern formed by their spatial configuration.