Long Yan, Huang He
Bone Marrow Transplantation Center, The First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, 310003, China.
Sci China Life Sci. 2015 Dec;58(12):1256-61. doi: 10.1007/s11427-015-4976-3. Epub 2015 Nov 26.
Hematopoietic stem cells (HSCs) are specified and generated during the embryonic development and have remarkable potential to replenish the full set of blood cell lineages. Researchers have long been interested in clarifying the molecular events involved in HSC specification. Many studies have reported the development of methods for generating functional hematopoietic cells from pluripotent stem cells (PSCs-embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs)) for decades. However, the generation of HSCs with robust long-term repopulation potential remains a swingeing challenge, of which a major factor contributing to this failure is the difficulty to define the intraembryonic signals related to the specification of HSCs. Since HSCs directly derive from hemogenic endothelium, in this review, we summarize both in vivo and in vitro studies on conserved signaling pathways that control the specification of HSCs from hemogenic endothelial cells.
造血干细胞（HSCs）在胚胎发育过程中被特化并产生，具有显著的潜力来补充全套血细胞谱系。长期以来，研究人员一直致力于阐明参与造血干细胞特化的分子事件。几十年来，许多研究报道了从多能干细胞（PSC——胚胎干细胞（ESCs）和诱导多能干细胞（iPSCs））生成功能性造血细胞的方法的进展。然而，生成具有强大长期重建潜力的造血干细胞仍然是一个巨大的挑战，导致这一失败的一个主要因素是难以确定与造血干细胞特化相关的胚胎内信号。由于造血干细胞直接来源于造血内皮细胞，在本综述中，我们总结了关于控制从造血内皮细胞特化造血干细胞的保守信号通路的体内和体外研究。
