Saxena Pooja, Thuenemann Eva C, Sainsbury Frank, Lomonossoff George P
Department of Chemistry, Indiana University, Bloomington, IN, USA.
Department of Biological Chemistry, John Innes Centre, Norwich Research Park, Norwich, UK.
Methods Mol Biol. 2016;1385:39-54. doi: 10.1007/978-1-4939-3289-4_3.
This chapter constitutes a practical guide to using the "pEAQ" vector series for transient or stable expression of one or more protein(s) in Nicotiana benthamiana plants. The pEAQ vectors are a series of small binary vectors designed for controlled expression of multiple proteins in plants. To achieve high levels of expression, an expression system based on translational enhancement by the untranslated regions of RNA-2 from cowpea mosaic virus (CPMV), named CPMV-HT, is used. The expression vector pEAQ-HT combines the user-friendly pEAQ plasmid with CPMV-HT to provide a system for high-level expression of proteins in plants.
本章构成了一份实用指南，介绍如何使用“pEAQ”载体系列在本氏烟草植株中瞬时或稳定表达一种或多种蛋白质。pEAQ载体是一系列小型双元载体，设计用于在植物中可控表达多种蛋白质。为实现高水平表达，使用了一种基于豇豆花叶病毒（CPMV）RNA-2非翻译区进行翻译增强的表达系统，即CPMV-HT。表达载体pEAQ-HT将用户友好型pEAQ质粒与CPMV-HT相结合，提供了一种在植物中高水平表达蛋白质的系统。
