Oliveira Joao Batista A, Vagnini Laura D, Petersen Claudia G, Renzi Adriana, Oliveira-Pelegrin Gabriela R, Mauri Ana L, Ricci Juliana, Massaro Fabiana C, Dieamant Felipe, Cavagna Mario, Baruffi Ricardo L R, Franco Jose G
Center for Human Reproduction Prof Franco Jr., Ribeirao Preto, SP, Brazil; Paulista Center for Diagnosis Research and Training, Ribeirao Preto, SP, Brazil.
Paulista Center for Diagnosis Research and Training, Ribeirao Preto, SP, Brazil.
Reprod Biomed Online. 2016 Jan;32(1):66-78. doi: 10.1016/j.rbmo.2015.09.018. Epub 2015 Oct 23.
Certain gene polymorphisms are associated with implantation failure and pregnancy loss. Studies of leukaemia inhibitory factor (LIF) gene polymorphisms are scarce. The LIF single nucleotide polymorphism (SNP) thymine (T)/guanine (G) (rs929271) was studied in women to determine whether an association existed with pregnancy outcomes after intracytoplasmic sperm injection (ICSI); 411 women who underwent ICSI were recruited. DNA was extracted from the peripheral blood, and the LIF gene SNP T/G (rs929271) was genotyped using real-time polymerase chain reaction. Participants were divided into three groups according to their LIF genotype: T/T (n = 168), T/G (n = 202) and G/G (n = 41). All IVF and ICSI procedures were carried out under the same clinical and laboratory conditions. The ICSI cumulative results (from fresh plus frozen cycles) of each genotype group were analysed. The G/G genotype in women was associated with a higher implantation rate (T/T: 15.9%, T/G: 16.2%, G/G: 27.0%; P < 0.05), ongoing pregnancy rate/patient (T/T: 31.5%, T/G: 36.1%, G/G: 53.7%; P < 0.05) and ongoing pregnancy rate/transfer (T/T: 18.5%, T/G: 20.2%, G/G: 36.7%; P < 0.05). LIF SNP T/G (rs929271) seems to be a susceptibility biomarker capable of predicting implantation efficiency and pregnancy outcomes.
某些基因多态性与植入失败和妊娠丢失有关。关于白血病抑制因子（LIF）基因多态性的研究较少。对女性进行了LIF单核苷酸多态性（SNP）胸腺嘧啶（T）/鸟嘌呤（G）（rs929271）的研究，以确定其与卵胞浆内单精子注射（ICSI）后妊娠结局是否存在关联；招募了411例行ICSI的女性。从外周血中提取DNA，采用实时聚合酶链反应对LIF基因SNP T/G（rs929271）进行基因分型。根据LIF基因型将参与者分为三组：T/T（n = 168）、T/G（n = 202）和G/G（n = 41）。所有体外受精和ICSI程序均在相同的临床和实验室条件下进行。分析了各基因型组的ICSI累积结果（来自新鲜周期加冷冻周期）。女性中的G/G基因型与较高的植入率（T/T：15.9%，T/G：16.2%，G/G：27.0%；P < 0.05）、患者持续妊娠率（T/T：31.5%，T/G：36.1%，G/G：53.7%；P < 0.05）和移植持续妊娠率（T/T：18.5%，T/G：20.2%，G/G：36.7%；P < 0.05）相关。LIF SNP T/G（rs929271）似乎是一种能够预测植入效率和妊娠结局的易感性生物标志物。
