Chattoraj Shyamtanu, Amin Asif, Jana Batakrishna, Mohapatra Saswat, Ghosh Surajit, Bhattacharyya Kankan
Department of Physical Chemistry, Indian Association for the Cultivation of Science, Jadavpur, Kolkata, 700 032, India), Fax.
Organic & Medicinal Chemistry Division, CSIR-Indian Institute of Chemical Biology, Jadavpur, Kolkata, 700 032, India.
Chemphyschem. 2016 Jan 18;17(2):253-9. doi: 10.1002/cphc.201500982. Epub 2015 Dec 4.
Fluorescent gold nanoclusters (AuNCs) capped with lysozymes are used to deliver the anticancer drug doxorubicin to cancer and noncancer cells. Doxorubicin-loaded AuNCs cause the highly selective and efficient killing (90 %) of breast cancer cells (MCF7) (IC50 =155 nm). In contrast, the killing of the noncancer breast cells (MCF10A) by doxorubicin-loaded AuNCs is only 40 % (IC50 =4500 nm). By using a confocal microscope, the fluorescence spectrum and decay of the AuNCs were recorded inside the cell. The fluorescence maxima (at ≈490-515 nm) and lifetime (≈2 ns), of the AuNCs inside the cells correspond to Au10-13 . The intracellular release of doxorubicin from AuNCs is monitored by Förster resonance energy transfer (FRET) imaging.
用溶菌酶包覆的荧光金纳米团簇（AuNCs）将抗癌药物阿霉素递送至癌细胞和非癌细胞。负载阿霉素的AuNCs可高效、高选择性地杀死90%的乳腺癌细胞（MCF7）（半数抑制浓度[IC50]=155纳米）。相比之下，负载阿霉素的AuNCs对非癌性乳腺细胞（MCF10A）的杀伤率仅为40%（IC50=4500纳米）。通过共聚焦显微镜记录细胞内AuNCs的荧光光谱和衰减情况。细胞内AuNCs的荧光最大值（约490 - 515纳米）和寿命（约2纳秒）对应于Au10 - 13。通过荧光共振能量转移（FRET）成像监测阿霉素从AuNCs的细胞内释放情况。
